A combination of two human neutralizing antibodies prevents SARS-CoV-2 infection in rhesus macaques

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   ScreenIT

   Oct 1, 2021

   SciScore for 10.1101/2021.09.27.462074: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Field Sample Permit: Studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the Bioqual Institutional Animal Care and Use Committee (IACUC). IACUC: Studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the Bioqual Institutional Animal Care and Use Committee (IACUC).
   Sex as a biological variable	Cynomolgus monkeys (Macaca fascicularis) (2.2 – 5.8 kg body weight; 6 to 12 years old) were mixed male and female and randomly assigned to groups.
   Randomization	Cynomolgus monkeys (Macaca fascicularis) (2.2 – 5.8 kg body weight; 6 to 12 years old) were mixed male and female and randomly …

   SciScore for 10.1101/2021.09.27.462074: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Field Sample Permit: Studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the Bioqual Institutional Animal Care and Use Committee (IACUC). IACUC: Studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the Bioqual Institutional Animal Care and Use Committee (IACUC).
   Sex as a biological variable	Cynomolgus monkeys (Macaca fascicularis) (2.2 – 5.8 kg body weight; 6 to 12 years old) were mixed male and female and randomly assigned to groups.
   Randomization	Cynomolgus monkeys (Macaca fascicularis) (2.2 – 5.8 kg body weight; 6 to 12 years old) were mixed male and female and randomly assigned to groups.
   Blinding	These studies were not blinded.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Plates were washed and sequentially incubated with an oligoclonal pool of SARS2-2, SARS2-11, SARS2-16, SARS2-31, SARS2-38, SARS2-57 and SARS2-71 anti-S (VanBlargan et al., 2021) antibodies and HRP-conjugated goat anti-mouse IgG (Sigma, 12-349) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin.	SARS2-38 suggested: None SARS2-57 suggested: None anti-S suggested: None anti-mouse IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, stably transfected CHO cells expressing either COV2-2130-YTE-LALA or COV2-2381-YTE-LALA were generated using Leap-In transposon vectors (ATUM) containing the respective antibody heavy and light chain genes and a glutamine synthetase gene as a selectable marker.	CHO suggested: None
   The viral stocks were expanded using Vero E6 cells and harvested on day 5 following inoculation.	Vero E6 suggested: RRID:CVCL_XD71)
   Antibody–virus complexes were added to Vero-TMPRSS2 cell monolayers in 96-well plates and incubated at 37 °C for 1 h.	Vero-TMPRSS2 suggested: JCRB Cat# JCRB1818, RRID:CVCL_YQ48)
   Software and Algorithms
   Sentences	Resources
   The time-weighted average (TWA) values for the change of sgRNA viral load in BAL or NP from day 1 to day 10 after viral challenge were calculated as the area under the curve (AUC) of the change in viral load in Prism (version 9.1.2; GraphPad) and then divided by 10 as described previously (Baum et al., 2020a) (Table S1).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   The fitting curves and confidence intervals to visualize the relationship between TWA and antibody levels were estimated using Lowess curve smoothing method using ggplot2 in R software.	ggplot2 suggested: (ggplot2, RRID:SCR_014601)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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2. 

   Version published to 10.1101/2021.09.27.462074 on bioRxiv

   Sep 29, 2021