1. SciScore for 10.1101/2021.09.03.21262611: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    NIH rigor criteria are not applicable to paper type.

    Table 2: Resources

    Experimental Models: Organisms/Strains
    For instance, in the case of a gene belonging to an autosome with 2 common variants (named A and B), 3 combinations are possible (A, B, and AB), and (consequently) 3 Boolean features were defined both for the AD and AR model.
    suggested: RRID:BDSC_203)
    Software and Algorithms
    Library enrichment was tested by qPCR, and the size distribution and concentration were determined using Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA).
    Agilent Bioanalyzer
    suggested: None
    Variant calling was performed according to the GATK4[24] best practice guidelines, using BWA [25] for mapping and ANNOVAR [26] for annotating.
    suggested: (BWA, RRID:SCR_010910)
    suggested: (ANNOVAR, RRID:SCR_012821)
    Finally, annotation was performed using Variant Effect Predictor (VEP, version 101).
    Variant Effect Predictor
    suggested: None
    Variants were genotyped with the GATK GenotypeGVCFs tool v4.1.8.1.
    suggested: (GATK, RRID:SCR_001876)
    Therefore, the genetic ancestry of the patients was estimated using a random forest classifier trained on samples from the 1000 genomes project and using as input features the first 20 principal components computed from the common variants by PLINK [27].
    suggested: (PLINK, RRID:SCR_001757)
    ABS(meanβ)∗count∗F Pathway enrichment analysis was made using the GSEA-preranked module (v. 7.2.4) of the Genepattern platform [26], on several pathway categories (BIOCARTA,
    suggested: (GenePattern, RRID:SCR_003201)
    KEGG, REACTOME, GOBP, HALLMARKS, C7 and C8), limiting the size of genesets to the 10-300 range and performing 10,000 permutations.
    suggested: (KEGG, RRID:SCR_012773)
    The networks showing similarity of significant pathways were built using the EnrichmentMap algorithm [30] in the Cytoscape suite (v. 3.8.2) [31–32].
    suggested: (EnrichmentMap, RRID:SCR_016052)
    suggested: (Cytoscape, RRID:SCR_003032)

    Results from OddPub: Thank you for sharing your code.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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