A Highly Potent SARS-CoV-2 Blocking Lectin Protein
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SciScore for 10.1101/2021.07.22.453309: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: The experiments with C57BL/6 and IFNAR-/-mice were performed with permission of the Ankara University Ethical Committee for Animal Experiments (06 May 2020, 20120-8-66) in the high containment animal facility (ABSL3+), conducted according to the national regulations on the operation and procedure of animal experiments’ ethics committees (regulation no. 26220, 9 September 2006).
IRB: The experiments with C57BL/6 and IFNAR-/-mice were performed with permission of the Ankara University Ethical Committee for Animal Experiments (06 May 2020, 20120-8-66) in the high containment animal facility (ABSL3+), conducted according to the national regulations on the operation and procedure of animal …SciScore for 10.1101/2021.07.22.453309: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: The experiments with C57BL/6 and IFNAR-/-mice were performed with permission of the Ankara University Ethical Committee for Animal Experiments (06 May 2020, 20120-8-66) in the high containment animal facility (ABSL3+), conducted according to the national regulations on the operation and procedure of animal experiments’ ethics committees (regulation no. 26220, 9 September 2006).
IRB: The experiments with C57BL/6 and IFNAR-/-mice were performed with permission of the Ankara University Ethical Committee for Animal Experiments (06 May 2020, 20120-8-66) in the high containment animal facility (ABSL3+), conducted according to the national regulations on the operation and procedure of animal experiments’ ethics committees (regulation no. 26220, 9 September 2006).Sex as a biological variable not detected. Randomization A total of 28 IFNAR-/- mice (8-12 weeks) were randomly divided into 6 groups for prophylaxis and contact experiments as well as controls Individuals in the prophylaxis group received intranasal rGRFT and inoculated with SARS-CoV-2 Ank1 after 4 hours. Blinding not detected. Power Analysis The suspension was sonicated at 30% power for 10 cycles of 15s on/45s off. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Then the membrane was incubated in 5% milk in TBS-T containing 1:10000 primary anti-his mouse antibody at 4°C, overnight. anti-hissuggested: NoneSecondary antibody solution containing 1:3000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) in PBS-T was added to each well and incubated for 1 hour. anti-mousesuggested: NoneThe x6-His peptide present in the recombinant protein was targeted as the control, detected using mouse anti-His antibodies, anti-mouse-IgG-HRP and conjugate and TMB substrate. anti-mouse-IgG-HRPsuggested: NoneExperimental Models: Cell Lines Sentences Resources A 150 µL of the mixture was then inoculated on Vero E6 Cells grown in a 96-well flat-bottomed tissue culture plate with highest concentration (6 µM) of rGRFT without the virus was involved as toxicity, serum-free DMEM as cell and 75 µL 100 TCID50 virus as virus controls in each plate. Vero E6suggested: RRID:CVCL_XD71)Experimental Models: Organisms/Strains Sentences Resources The experiments with C57BL/6 and IFNAR-/-mice were performed with permission of the Ankara University Ethical Committee for Animal Experiments (06 May 2020, 20120-8-66) in the high containment animal facility (ABSL3+), conducted according to the national regulations on the operation and procedure of animal experiments’ ethics committees (regulation no. 26220, 9 September 2006). C57BL/6suggested: NoneA total of 28 IFNAR-/- mice (8-12 weeks) were randomly divided into 6 groups for prophylaxis and contact experiments as well as controls Individuals in the prophylaxis group received intranasal rGRFT and inoculated with SARS-CoV-2 Ank1 after 4 hours. IFNAR-/-suggested: NoneRecombinant DNA Sentences Resources grft gene was cloned into the pet22b vector without a pelb leader sequence, using Gibson assembly. pet22bsuggested: RRID:Addgene_84863)Software and Algorithms Sentences Resources Graphs were generated via GraphPad Prism software. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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