Increased LPS levels coexist with systemic inflammation and result in monocyte activation in severe COVID-19 patients

Article activity feed

1. Version published to 10.1016/j.intimp.2021.108125

   Nov 1, 2021
2. 

   ScreenIT

   Jul 1, 2021

   SciScore for 10.1101/2021.06.24.21259468: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: The study was approved by the UFCSPA Ethics Committee (CAAE: 38886220.0.0000). Consent: Informed consent was obtained from those legally responsible for the patients after the procedures had been explained.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Then, cells were labelled with monoclonal antibodies (all anti-human) conjugated with specific fluorochromes: CD14 Fitc, CD16 Pe, TLR4 Pe, CD69 Percp-Cy5.5, CD192 (CCR2) Pe, CD195 (CCR5), CD197 (CCR7) Fitc, HLA-DR PercP-Cy5.5 (all from EbioScience, ThermoFisher, USA).	anti-human suggested: …

   SciScore for 10.1101/2021.06.24.21259468: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: The study was approved by the UFCSPA Ethics Committee (CAAE: 38886220.0.0000). Consent: Informed consent was obtained from those legally responsible for the patients after the procedures had been explained.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Then, cells were labelled with monoclonal antibodies (all anti-human) conjugated with specific fluorochromes: CD14 Fitc, CD16 Pe, TLR4 Pe, CD69 Percp-Cy5.5, CD192 (CCR2) Pe, CD195 (CCR5), CD197 (CCR7) Fitc, HLA-DR PercP-Cy5.5 (all from EbioScience, ThermoFisher, USA).	anti-human suggested: None CD14 suggested: (BioLegend Cat# 348805, RRID:AB_2889063) CD16 suggested: None TLR4 suggested: None CD69 suggested: (GenWay Biotech Inc. Cat# GWB-EA1DA7, RRID:AB_10269678) CD192 suggested: (GeneTex Cat# GTX10396, RRID:AB_373990) CCR2 suggested: (GeneTex Cat# GTX10396, RRID:AB_373990) CD195 suggested: None CD197 suggested: None CCR7 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   THP-1 were identified and gated according to each forward scatter (FSC) and side scatter (SSC) profile and the mean fluorescence intensity (MFI) was evaluated.	THP-1 suggested: CLS Cat# 300356/p804_THP-1, RRID:CVCL_0006)
   Software and Algorithms
   Sentences	Resources
   The SPSS 22.0 (IBM Inc, EUA) software was used in all analysis.	SPSS suggested: (SPSS, RRID:SCR_002865)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.06.24.21259468 on medRxiv

   Jun 27, 2021