Dual roles of a novel oncolytic viral vector-based SARS-CoV-2 vaccine: preventing COVID-19 and treating tumor progression

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   ScreenIT

   Jun 10, 2021

   SciScore for 10.1101/2021.06.07.447286: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Each patient had signed a consent as part of the IRB-approved protocol 20126 at City of Hope. IACUC: Ethics statement: All experiments using mice were conducted in compliance with federal, state, and local guidelines and with approval from the City of Hope Animal Care and Use Committee.
   Sex as a biological variable	In vivo mouse model: Six-to eight-week-old female BALB/c or C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, Maine).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	Contamination: All cell lines were routinely tested for the absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza …

   SciScore for 10.1101/2021.06.07.447286: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Each patient had signed a consent as part of the IRB-approved protocol 20126 at City of Hope. IACUC: Ethics statement: All experiments using mice were conducted in compliance with federal, state, and local guidelines and with approval from the City of Hope Animal Care and Use Committee.
   Sex as a biological variable	In vivo mouse model: Six-to eight-week-old female BALB/c or C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, Maine).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	Contamination: All cell lines were routinely tested for the absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza (Walkersville, MD).

   Table 2: Resources

   Antibodies
   Sentences	Resources
   SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody, followed by detecting with an anti-rabbit secondary antibody (Cat. # ab6721, Abcam) at a 1:20,000 dilution.	SARS-CoV-2 viral nucleocapsid protein (NP) suggested: None SARS-CoV-2 viral nucleocapsid protein (NP suggested: None anti-NP protein suggested: (Creative Diagnostics Cat# DPAB-DC4728, RRID:AB_2501850) anti-rabbit suggested: (Abcam Cat# ab6721, RRID:AB_955447)
   Mouse anti-spike antibody was diluted at 1:1000 in PBST containing 1% BSA and incubated with the membrane at 4°C overnight.	anti-spike suggested: None
   HRP-conjugated goat anti-mouse IgG antibody (Cat. #05-4220, Invitrogen) was used for detection.	anti-mouse IgG suggested: None
   After treatment with brefeldin A (Cat. # 420601, Biolegend) for 4 hours, the splenocytes were stained with the extracellular markers PE-Cy ™ 7 Hamster Anti-Mouse CD3e (Cat. # 552774, BD Pharmingen), APC-Cy7 Rat Anti-Mouse CD4 (Cat. # 552051, BD Pharmingen), and CD8 alpha Monoclonal Antibody (KT15), FITC (Cat. # MA5-16759.	PE-Cy suggested: None Anti-Mouse CD3e suggested: None CD4 suggested: (BD Biosciences Cat# 552051, RRID:AB_394331) CD8 suggested: (Thermo Fisher Scientific Cat# MA5-16759, RRID:AB_2538242)
   Intravenous injection of OV-spike induces anti-S antibody production in mouse sera.	anti-S suggested: None
   No significant difference of anti-S-specific antibody production between the tumor models. Fig. S6.	anti-S-specific suggested: None S6 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Vero cells were used for propagating and titrating the viruses.	Vero suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
   Virus isolates were passaged in Vero E6 cells (ATCC CRL-1586) as previously described (47).	Vero E6 suggested: None
   The B16 melanoma mouse model was established by injecting 5×105 B16 cells s.c. 5 days before OV or saline injection into C57BL/6 mice.	B16 suggested: None
   The MC38 and ID8 colon adenocarcinoma and ovarian cancer mouse models were established by injecting 5×105 MC38 cells or 1×106 ID8 cells i.p. 4 days before OV or saline injection into C57BL/6 mice.	MC38 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   In vivo mouse model: Six-to eight-week-old female BALB/c or C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, Maine).	C57BL/6 suggested: None
   Histopathology: Mice organs including lung, brain, kidney, and liver were freshly isolated from 1×106 pfu OV-spike-, OV-Q1- or saline i.v. injected BALB/c mice on day 70 post injection.	BALB/c suggested: None
   Recombinant DNA
   Sentences	Resources
   The fusion protein was inserted into pT-oriSIE4/5 following the HSV pIE4/5 promoter to construct pT-oriSIE4/5-spike.	pT-oriSIE4/5 suggested: None pT-oriSIE4/5-spike suggested: None
   Software and Algorithms
   Sentences	Resources
   Flow cytometry data were acquired on a BD LSRFortessa X-20 (BD) and analyzed by FlowJo software.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   Statistical analysis: Prism software v.8 (GraphPad, CA, USA) and SAS v.	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Version published to 10.1101/2021.06.07.447286v1 on bioRxiv

   Jun 7, 2021