Topic: Hyper immune Bovine Colostrum as a Low-Cost, Large-Scale Source of Antibodies against COVID-19
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Abstract
Many different strategies have been used to fight against COVID-19 pandemic as a therapeutics or prophylaxis approaches. However, none of them so far have used, passive immune transfer using products from immunized farm animals. Hyper immune bovine colostrums (HBC) have been used against many different respiratory and gastrointestinal tracts infections during past decades.
Six mixed Holstein X Semental dairy cattle’s in their 6-7 months of gestation period years were chosen for hyper immunization with COVID-19 vaccine. An isolated and very well protected site was selected and equipped according to animal husbandry code of practice, used for animal experimentation. Specific IgG level against SARS-CoV-2 virus was measured before and after vaccination in the sera, and in the colostrum following parturition. Very high specific IgG level was detected one week following second vaccination in the sera and in first colostrums after parturition. Safety of the product was approved following phase 1 of clinical trials in 40 healthy volunteers. Phase 2 of the clinical trials is underway. Early results show effectiveness of the product in reducing sore throat and cough in early stages of SARS-CoV-2 infection.
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SciScore for 10.1101/2021.05.20.444932: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Blood samples were collected on weekly intervals from milk vein of the animal into special serum tubes, kept in room temperature for 30 min to coagulate red blood cells, and then centrifuged at 2500 g for 15 min at room temperature. Sex as a biological variable Clinical observation: Before and after vaccination, pregnant cows were closely monitored on daily basis for any changes in behavior such as occasional systemic shock, itching, swelling or any adverse effects in the vaccination site in the thigh muscle. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources We just … SciScore for 10.1101/2021.05.20.444932: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Blood samples were collected on weekly intervals from milk vein of the animal into special serum tubes, kept in room temperature for 30 min to coagulate red blood cells, and then centrifuged at 2500 g for 15 min at room temperature. Sex as a biological variable Clinical observation: Before and after vaccination, pregnant cows were closely monitored on daily basis for any changes in behavior such as occasional systemic shock, itching, swelling or any adverse effects in the vaccination site in the thigh muscle. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used. anti-bovine IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Briefly, the virus was isolated from the naso-pharynx samples taken from COVID-19 positive patients, cultured on WHO Vero cell line. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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