Antibody Cocktail Exhibits Broad Neutralization against SARS-CoV-2 and SARS-CoV-2 variants

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   ScreenIT

   Apr 19, 2021

   SciScore for 10.1101/2021.04.16.440083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	The library’s initial diversity was evaluated and assured by sequencing of randomly picked clones for each step of library construction and the complexity of the library was then calculated.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After washing with PBST, horseradish peroxidase (HRP)-conjugated anti-human IgG antibody (Sigma, USA) was added at the dilution of 1:20000 and incubated at 37°C for 30min.	30min suggested: None
   Then the cells were incubated with 20 μg/ml mAbs or isotype IgG mAb of hepatitis b virus …

   SciScore for 10.1101/2021.04.16.440083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	The library’s initial diversity was evaluated and assured by sequencing of randomly picked clones for each step of library construction and the complexity of the library was then calculated.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After washing with PBST, horseradish peroxidase (HRP)-conjugated anti-human IgG antibody (Sigma, USA) was added at the dilution of 1:20000 and incubated at 37°C for 30min.	30min suggested: None
   Then the cells were incubated with 20 μg/ml mAbs or isotype IgG mAb of hepatitis b virus (HBV), at room temperature for 1 h, followed by further incubation with anti-human IgG FITC-conjugated antibody (Sigma, USA).	HBV suggested: None
   Then cells were stained with anti-mouse IgG Taxes red-conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACSAria II (BD, USA)	anti-mouse IgG suggested: None anti-human IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cells and Viruses: Cell lines (HEK293T and VeroE6 cells) were initially acquired from the American Type Culture Collection (ATCC; USA).	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   EXPi293F cells were purchased from Life Technologies, USA.	EXPi293F suggested: RRID:CVCL_D615)
   Afterward, HuH-7 cells were added into the plates (2×104 cells/100 µl per well), followed by 24h incubation at 37°C in a humidified atmosphere with 5% CO2.	HuH-7 suggested: None
   Then mixtures were added to 2.5 × 105 HEK293T cells expressing ACE2 and incubated at 4 °C for another hour.	HEK293T suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
   Software and Algorithms
   Sentences	Resources
   The data was analyzed using GraphPad Prism 8.0.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   The half-maximal inhibitory concentrations (IC50) were determined using 4-parameter logistic regression (GraphPad Prism version 8).	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   All of these data were analyzed using FlowJo.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   A suitable template was obtained through a BLAST search of the Protein Databank (PDB).	BLAST suggested: (BLASTX, RRID:SCR_001653)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 34, 27, 28 and 29. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2021.04.16.440083 on bioRxiv

   Apr 17, 2021