Two-Stage Hierarchical Group Testing Strategy to Increase SARS-CoV-2 Testing Capacity at an Institution of Higher Education
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SciScore for 10.1101/2021.04.14.21255494: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Sample Collection: The study was conducted at the Life Science Testing Center (LSTC; Burlington, MA, USA) for Northeastern University (NEU) students, faculty, and staff within the United States Northeast (MA, ME) regional campuses. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Multiplex RT-qPCR …
SciScore for 10.1101/2021.04.14.21255494: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Sample Collection: The study was conducted at the Life Science Testing Center (LSTC; Burlington, MA, USA) for Northeastern University (NEU) students, faculty, and staff within the United States Northeast (MA, ME) regional campuses. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Multiplex RT-qPCR has been sought to increase assay sensitivity for the detection of SARS-CoV-2, yet inherent limitations arise from increased competition in reaction kinetics [21]. The genomic architecture of SARS-CoV-2 exhibits extensive secondary structure with regions consisting of high base pairing content thought to be mechanistically advantageous for the transcription and thermodynamic stability of sub-genomic RNA [22]. Further consideration that retro-transcription occurs from the 3’ direction suggests cDNA production from the N gene with less deviation than the S and ORF1ab genes. Concurrent with our results, the N gene demonstrated the greatest stability within the negative sample matrix, which was confirmed by a limit of detection 1 and 3 orders of magnitude less than the ORF1ab and S genes, respectively. Recent identification of a SARS-CoV-2 variant of concern (VOC) 202012/01 has been associated with several mutations in the S gene including a deletion at position 69 and 70 to cause S-gene target failure by the TaqPath COVID-19 method [23]. Due to the enhanced binding interactions of VOC 202012/01 spike protein with the target angiotensin converting enzyme 2 (ACE-2), estimates suggest the increased transmissibility will permit strain predominance in the United States by spring 2021 [24]. While no genomic sequence data was available for the samples used in this study, it is important to consider the international nature of the university population as many Northeas...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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