Interactions between SARS-CoV-2 N-protein and α-synuclein accelerate amyloid formation
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Abstract
First cases that point at a correlation between SARS-CoV-2 infections and the development of Parkinson’s disease have been reported. Currently it is unclear if there also is a direct causal link between these diseases. To obtain first insights into a possible molecular relation between viral infections and the aggregation of α-synuclein protein into amyloid fibrils characteristic for Parkinson’s disease, we investigated the effect of the presence of SARS-CoV-2 proteins on α-synuclein aggregation. We show, in test tube experiments, that SARS-CoV-2 S-protein has no effect on α-synuclein aggregation while SARS-CoV-2 N-protein considerably speeds up the aggregation process. We observe the formation of multi-protein complexes, and eventually amyloid fibrils. Microinjection of N-protein in SHSY-5Y cells disturbed the α-synuclein proteostasis and increased cell death. Our results point toward direct interactions between the N-protein of SARS-CoV-2 and α-synuclein as molecular basis for the observed coincidence between SARS-CoV-2 infections and Parkinsonism.
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SciScore for 10.1101/2021.04.12.439549: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell culture, microinjection, and nuclear counterstaining: SH-SY5Y cells (ATCC, USA) were grown in proliferation medium (DMEM-F12 GlutaMAX™ + 10% heat inactivated FBS + 1% non-essential amino acids +10 mM HEPES buffer + 1% Penicillin/Streptomycin; Gibco®, Invitrogen, USA). SH-SY5Ysuggested: NoneSoftware and Algorithms Sentences Resources AFM images were processed with the Scanning Probe Image Processor (SPIP SPIPsuggested: (SPIP, RRID:SCR_009410)Fibril morphology was analyzed using a custom fibril analysis Matlab script adapted from the FiberApp package. Matlabsuggested: (MATLAB, RRID:SC…SciScore for 10.1101/2021.04.12.439549: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell culture, microinjection, and nuclear counterstaining: SH-SY5Y cells (ATCC, USA) were grown in proliferation medium (DMEM-F12 GlutaMAX™ + 10% heat inactivated FBS + 1% non-essential amino acids +10 mM HEPES buffer + 1% Penicillin/Streptomycin; Gibco®, Invitrogen, USA). SH-SY5Ysuggested: NoneSoftware and Algorithms Sentences Resources AFM images were processed with the Scanning Probe Image Processor (SPIP SPIPsuggested: (SPIP, RRID:SCR_009410)Fibril morphology was analyzed using a custom fibril analysis Matlab script adapted from the FiberApp package. Matlabsuggested: (MATLAB, RRID:SCR_001622)FiberAppsuggested: (FiberApp, RRID:SCR_015826)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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