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  1. Reviewer 2. Professor.Gong Zhang

    Is the language of sufficient quality? No.

    Are all data available and do they match the descriptions in the paper?

    No. Hi-C data was not deposited.

    Is the data acquisition clear, complete and methodologically sound?

    No. The quality of the nanopore sequencing datasets was not evaluated. The error correction using short-read sequencing was not clear. It seems not necessary to use Hi-C data for the assembly.

    Is there sufficient detail in the methods and data-processing steps to allow reproduction? No. Error correction was not clear.

    Is there sufficient data validation and statistical analyses of data quality? No.

    Is the validation suitable for this type of data?

    No. No validation of the variants was performed. The authors used multiple SNV detection algorithms and got quite different results. They should experimentally validate which one is better.

    Is there sufficient information for others to reuse this dataset or integrate it with other data?

    No. It is difficult to reuse it. There's little annotation done.

    Additional Comments: I don't understand why the authors chose to sequence a woman. As a reference of a certain ethnic, complete chromosomes are needed, which means a man (XY) is necessary.

  2. This work has been published in GigaByte Journal under a CC-BY 4.0 license (, and has published the reviews under the same license. These are as follows.

    Reviewer 1. Dr.Giulio Formenti

    First review: Language: A few minor typos to correct, highlighted in the revised manuscript

    Is there sufficient detail in the methods and data-processing steps to allow reproduction?

    Yes, but please revise as per my comments

    Additional Comments: see comments here

    Decision: Minor Revision.


    I am happy with the changes and I think the article is worth publishing in Gigabytes. However, I think one main point needs further clarity. Since this is mostly about a new dataset and assembly, the authors should make it very clear to the reader what they did. I think the title is still misleading in this respect. In it the authors refer to an "assembly with short and long reads combined with Hi-C data". This is not how one would generally refer to such an assembly in the community as it reads as if a short-read based assembly was complemented with long reads (gap filling?) and hic reads (phasing?). I suggest rephrasing as "a ONT long-read-based assembly scaffolded with Hi-C data and polished with short reads". The confusion/ambiguity about this is further reinforced in the text. I think the authors should make and extra effort reading the text to make sure the genome assembly terminology is consistent with the state of the art and therefore very clear to the reader. For instance, in the abstract the authors say that the assembly was constructed using 57x ultra-long nanopore reads. I think this is incorrect. Ultra-long nanopore reads are usually defined as reads >100kbp. I don't think the authors filtered their dataset for ultralong and this should be corrected. Indeed, it would be interesting to know what fraction of ultralong reads are available in their 57x dataset.