Increased viral variants in children and young adults with impaired humoral immunity and persistent SARS-CoV-2 infection: A consecutive case series

Article activity feed

1. Version published to 10.1016/j.ebiom.2021.103355

   May 1, 2021
2. 

   ScreenIT

   Mar 15, 2021

   SciScore for 10.1101/2021.02.27.21252099: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: 11 The study was approved by the CHLA Institutional Review Board (CHLA-16-00429).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Case histories: Patient 1 is a previously healthy female under 5 years of age with no significant past medical history prior to several admissions to the emergency department (ED) for worsening pancytopenia, decreased appetite, and abdominal pain.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Measurement of anti-SARS-CoV-2 antibodies in serum: IgG to the S1 domain of SARS-CoV-2 spike was measured from serum within 24 hours of collection …

   SciScore for 10.1101/2021.02.27.21252099: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: 11 The study was approved by the CHLA Institutional Review Board (CHLA-16-00429).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Case histories: Patient 1 is a previously healthy female under 5 years of age with no significant past medical history prior to several admissions to the emergency department (ED) for worsening pancytopenia, decreased appetite, and abdominal pain.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Measurement of anti-SARS-CoV-2 antibodies in serum: IgG to the S1 domain of SARS-CoV-2 spike was measured from serum within 24 hours of collection with a commercial enzyme-linked immunosorbent assay (ELISA) kit (EUROIMMUN, Lubeck, Germany).	anti-SARS-CoV-2 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   11 Viral culture: Viral culture was performed using SARS-CoV-2-susceptible VeroE6TMPRSS2 cells15,16 adapted from the VeroE6 cell line (ATCC CRL-1586) to express the TMPRSS2 protease at levels approximately 10-fold higher than that found in the human lung.	VeroE6 suggested: None
   150 µL of clinical specimen was used to inoculate one well of a 24 well plate of VeroE6TMPRSS2 cells as previously described.	VeroE6TMPRSS2 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Software and Algorithms
   Sentences	Resources
   Viral genome library construction and sequencing: Whole-genome sequencing (WGS) libraries of viral cDNA were prepared as previously described using the CleanPlex SARS-CoV-2 Research and Surveillance NGS Panel (Paragon Genomics, Hayward, CA).	WGS suggested: None
   Paragon and Twist nucleotide sequences were aligned with NovoAlign (Novocraft Technologies, Selengor, Malaysia) aligner.	NovoAlign suggested: (NovoAlign, RRID:SCR_014818)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   One of the limitations of a small case series is the generalizability of findings and more work is needed to better understand the many host factors that can influence viral clearance and mutation within these patients. However, we conclude that the observed expanded intra-host heterogeneity of viral isolates from pediatric and young adult patients with suppressed immunity and correspondingly prolonged infection raises the possibility of emergence of variants with the potential to evade immune responses elicited during the course of infection or induced by vaccine. This possibility warrants further study and, in the meantime, serious consideration of extended infection control precautions and genomic monitoring in this patient population.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2021.02.27.21252099v1 on medRxiv

   Mar 2, 2021