GABA administration limits viral replication and pneumonitis in a mouse model of COVID-19

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   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.02.09.430446: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: The protocols for all experiments using vertebrate animals were approved by the Animal Research Committee at UCLA (approval # ARC-2020-122) and were carried out in compliance with the ARRIVE guidelines.
   Randomization	The mice were immediately randomized and provided with plain water (controls) or water that contained GABA (20 mg/mL) as per (9, 39) for the entirety of the observation period.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Mice: Female A/J mice (8 weeks in age) were purchased from the Jackson Laboratory and maintained in microisolator cages and fed with a standard diet and water ad libitum.
   Cell Line Authentication	not…

   SciScore for 10.1101/2021.02.09.430446: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: The protocols for all experiments using vertebrate animals were approved by the Animal Research Committee at UCLA (approval # ARC-2020-122) and were carried out in compliance with the ARRIVE guidelines.
   Randomization	The mice were immediately randomized and provided with plain water (controls) or water that contained GABA (20 mg/mL) as per (9, 39) for the entirety of the observation period.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Mice: Female A/J mice (8 weeks in age) were purchased from the Jackson Laboratory and maintained in microisolator cages and fed with a standard diet and water ad libitum.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus: MHV-1, DBT cells, and HeLa-CECAM1 were generously provided by Dr. Stanley Perlman (University of Iowa).	HeLa-CECAM1 suggested: None
   The viral titers in the supernatants were determined by endpoint dilution (40) in HeLa-CEACAM1 cells (85% confluent, 5 × 104 cells/well) using the Spearman-Kärber formula (41) to calculate 50% tissue culture infectious dose (TCID50).	HeLa-CEACAM1 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Mice: Female A/J mice (8 weeks in age) were purchased from the Jackson Laboratory and maintained in microisolator cages and fed with a standard diet and water ad libitum.	A/J suggested: RRID:IMSR_JAX:000646)
   Software and Algorithms
   Sentences	Resources
   Reagents: GABA (stock #A2129) was purchased from Millipore-Sigma (St. Louis, MO, USA).	Millipore-Sigma suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
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   Version published to 10.1101/2021.02.09.430446 on bioRxiv

   Feb 9, 2021