Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat RBD of SARS-CoV-2 spike protein protects mice against COVID-19

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   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.02.05.429860: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Materials: Ethics statement: All animal experiments were approved by the Committee on the Ethics of Animal Experiments of the IMCAS, and conducted in compliance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the IMCAS Ethics Committee. IRB: Materials: Ethics statement: All animal experiments were approved by the Committee on the Ethics of Animal Experiments of the IMCAS, and conducted in compliance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the IMCAS Ethics Committee.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Female …

   SciScore for 10.1101/2021.02.05.429860: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Materials: Ethics statement: All animal experiments were approved by the Committee on the Ethics of Animal Experiments of the IMCAS, and conducted in compliance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the IMCAS Ethics Committee. IRB: Materials: Ethics statement: All animal experiments were approved by the Committee on the Ethics of Animal Experiments of the IMCAS, and conducted in compliance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the IMCAS Ethics Committee.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Female BALB/c mice at 6 to 8 weeks of age were immunized with 1 × 1011 vp of vaccine or sham control through the i.m. or i.n. route.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Protein samples were separated by 12% SDS-PAGE and analyzed by Western blotting with rabbit anti-RBD of SARS-CoV-2 polyclonal antibody.	anti-RBD suggested: None
   Goat anti-rabbit IgG-horseradish peroxidase (HRP) antibodies were used as secondary antibodies.	anti-rabbit IgG-horseradish peroxidase (HRP) suggested: None
   Plates were incubated with goat anti-mouse IgG-HRP antibody or goat anti-mouse IgA-HRP antibody and subsequently developed with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate.	anti-mouse IgG-HRP suggested: None anti-mouse IgA-HRP suggested: None
   The cells were subsequently fixed and permeabilized in permeabilizing buffer (BD Biosciences, USA) and stained with anti-mouse anti-IFNγ (BioLegend), anti-TNFα (BioLegend), anti-IL-2 (BioLegend), anti-IL-4 (BioLegend) and anti-IL-10 (BioLegend) antibodies.	anti-mouse anti-IFNγ ( BioLegend) suggested: None anti-TNFα suggested: None anti-IL-2 suggested: None anti-IL-4 suggested: None anti-IL-10 suggested: None
   Besides, tissue sections were stained with anti-SARS-CoV-2 nucleoprotein antibody (Sino Biological, China) to detect virus infection.	anti-SARS-CoV-2 nucleoprotein suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   These recombinant adenovirus genomes were linearized and transfected into HEK293 cells to rescue the recombinant adenovirus, which was further propagated and purified by cesium chloride density gradient centrifugation as previously described (88).	HEK293 suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)
   Western blot: HEK 293T cells were preplated in 6-well plate, followed by infected with 1 × 109 vp of AdC7-S, AdC7-RBD, AdC7-RBD-tr2 or AdC7-empty as sham control.	HEK 293T suggested: None
   Then the mixture was transferred to pre-plated Huh7 cell monolayers in 96-well plates.	Huh7 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Animal protection against virus challenge: To evaluate the protection efficacy of vaccine candidates against SARS-CoV-2, a recombinant adenovirus Ad5-hACE2 transducing BALB/c mice model was used.	BALB/c suggested: RRID:IMSR_ORNL:BALB/cRl)
   Software and Algorithms
   Sentences	Resources
   The coding sequence was codon-optimized for mammalian cell expression and synthesized by GENEWIZ, China.	GENEWIZ suggested: (GENEWIZ, RRID:SCR_003177)
   Graphs were generated with GraphPad Prism software.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2021.02.05.429860 on bioRxiv

   Feb 8, 2021
3. Version published to 10.1080/22221751.2021.1959270

   Jan 1, 2021