SARS-CoV-2 infection of circulating immune cells is not responsible for virus dissemination in severe COVID-19 patients

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1. 

   ScreenIT

   Jan 20, 2021

   SciScore for 10.1101/2021.01.19.427282: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: All experiments involving or human samples received approval from the Conjoint Health Ethics Review Board at the University of Calgary.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   To produce viral stocks, Vero E6 cells were infected at an MOI of 0.01 for 1h in serum-free DMEM (Thermo Fisher) at 33°C in a humidified incubator with 5% CO2.	Vero E6 suggested: None
   Pooled human umbilical cord vein endothelial cells (HUVECs, Lonza, Basal, Switzerland) were cultured using endothelial growth …

   SciScore for 10.1101/2021.01.19.427282: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: All experiments involving or human samples received approval from the Conjoint Health Ethics Review Board at the University of Calgary.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   To produce viral stocks, Vero E6 cells were infected at an MOI of 0.01 for 1h in serum-free DMEM (Thermo Fisher) at 33°C in a humidified incubator with 5% CO2.	Vero E6 suggested: None
   Pooled human umbilical cord vein endothelial cells (HUVECs, Lonza, Basal, Switzerland) were cultured using endothelial growth media (EGM-2; Lonza).	HUVECs suggested: None
   Software and Algorithms
   Sentences	Resources
   Dimensionality reduction, clustering and gene expression: Output aggregated, filtered feature matrix files were imported into a SeuratObject using Seurat V3.1.5 and R Version 3.6.1, using default parameters 28,34.	Seurat suggested: (SEURAT, RRID:SCR_007322)
   Liquid Chromatography and Mass Spectrometry (LC-MS/MS): Tryptic peptides were analyzed on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) operated with Xcalibur (version 4.0.21.10) and coupled to a Thermo Scientific Easy-nLC	Xcalibur suggested: (Thermo Xcalibur, RRID:SCR_014593)

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   Results from OddPub: Thank you for sharing your code and data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. 

   Version published to 10.1101/2021.01.19.427282v1 on bioRxiv

   Jan 19, 2021