Humoral and Cell-Mediated Immune Response in Colostrum from Women Diagnosed Positive for SARS-CoV-2
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SciScore for 10.1101/2021.01.03.20248715: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Recruitment of COVID-19-positive participants: Study participants were patients at UMass Memorial Medical Center (UMMC, Worcester, MA) and provided consent in accordance with an IRB-approved protocol (H00020140). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Selection of pre-COVID-19 controls: We identified archived samples from eight women who donated liquid bilateral colostrum (1-3 days post-partum) during June 2011-May 2013. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The receptor binding domain (RBD) spike protein cloned into the … SciScore for 10.1101/2021.01.03.20248715: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Recruitment of COVID-19-positive participants: Study participants were patients at UMass Memorial Medical Center (UMMC, Worcester, MA) and provided consent in accordance with an IRB-approved protocol (H00020140). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Selection of pre-COVID-19 controls: We identified archived samples from eight women who donated liquid bilateral colostrum (1-3 days post-partum) during June 2011-May 2013. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The receptor binding domain (RBD) spike protein cloned into the pCAGGS expression vector was expressed in HEK293T cells (ATCC) using PEI (10:1 PEI:DNA ratio) and purified by gravity flow, as described in Stadlbauer et al19. HEK293Tsuggested: NoneSoftware and Algorithms Sentences Resources Briefly, 96-well plates (Fisher Sci., #351172) were coated with the RBD spike protein at 1 μg/mL in 1X phosphate-buffered saline and incubated with gentle shaking overnight at 4°C, followed by blocking in 5% (w/v) dry skimmed milk in TBST with gentle shaking for 30 minutes at RT. Fisher Sci.suggested: (One Mind Biospecimen Bank Listing, RRID:SCR_004193)Plates were washed 3 times, incubated with 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ATBS; Sigma Aldrich, #A9941) diluted at 0.2 mg/mL in 0.1 M Sodium Acetate pH 4.5 at 370C for 30 minutes. ATBS; Sigma Aldrichsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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