Ferritin nanoparticle based SARS-CoV-2 RBD vaccine induces persistent antibody response and long-term memory in mice

  1. Wenjun Wang
  2. Baoying Huang
  3. Yanping Zhu
  4. Wenjie Tan
  5. Mingzhao Zhu

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Abstract

Since the outbreak of COVID-19, over 200 vaccine candidates have been documented and some of them have advanced to clinical trials with encouraging results. However, the antibody persistence over 3 months post immunization and the long-term memory have been rarely reported. Here, we report that a ferritin nanoparticle based SARS-CoV-2 RBD vaccine induced in mice an efficient antibody response which lasts for at least 7 months post immunization. Significantly higher number of memory B cells were maintained and a significantly higher level of recall response was induced upon antigen challenge. Thus, we believe our current study provide the first information about the long-term antibody persistence and memory response of a COVID-19 vaccine. This information would be also timely useful for the development and evaluation of other vaccines.

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  1. ScreenIT

    SciScore for 10.1101/2020.12.22.423894: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIACUC: All animal experiments were performed in accordance with the guidelines of the Institute of Biophysics, Chinese Academy of Sciences, using protocols approved by the Institutional Laboratory Animal Care and Use Committee.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Horseradish peroxidase (HRP) conjugated goat anti-mouse IgG (H+L) (1:5000, ZSGB-BIO) was used as second antibody.
    anti-mouse IgG
    suggested: (LSBio (LifeSpan Cat# LS-C69682-5000, RRID:AB_1653096)
    The antibodies used included anti-B220-PE (RA3-6B2, eBioscience), anti-CD38-APC (90, eBioscience), anti-IgM-PE/Cy7 (II/41, eBioscience) and anti-IgD-PerCP/Cy5.5 (11-26c.2a, Biolegend).
    anti-B220-PE
    suggested: None
    RA3-6B2
    suggested: None
    anti-CD38-APC
    suggested: (Sigma-Aldrich Cat# SAB4700171, RRID:AB_10896264)
    anti-IgM-PE/Cy7
    suggested: None
    anti-IgD-PerCP/Cy5.5
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Briefly, the sera were 2-fold serially diluted using 2% FBS-DMEM and mixed with the same volume of live SARS-CoV-2 (C-Tan-nCoV strain 04, 100TCID50), the mixtures were incubated at 37 °C for 1 h, following which they were added to the seeded Vero cells.
    Vero
    suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
    Experimental Models: Organisms/Strains
    SentencesResources
    Immunization: Female naïve WT C57BL/6 mice (8-9 weeks old) were subcutaneously immunized with 500 pmol (approximately 30.7 μg, as determined by single ferritin-RBD subunit) ferritin-NP-RBD vaccine or 500 pmol (approximately 13.7 μg) RBD-SpyTag with 30 μg CpG-1826
    C57BL/6
    suggested: None
    Software and Algorithms
    SentencesResources
    Statistical analysis: All analysis was performed using GraphPad Prism statistical software (GraphPad Software Inc.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 16. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1101/2020.12.22.423894 on bioRxiv