Decay of Fc-dependent antibody functions after mild to moderate COVID-19
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SciScore for 10.1101/2020.12.13.20248143: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study protocols were approved by the University of Melbourne Human Research Ethics Committee (#2056689).
Consent: All subjects provided written informed consent in accordance with the Declaration of Helsinki.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Convalescent donors either had a PCR+ test during early infection or clear exposure to SARS-CoV-2, and were confirmed to have SARS-CoV-2 S- and RBD-specific antibodies via ELISA as previously reported (1). SARS-CoV-2suggested: NoneTo stain for … SciScore for 10.1101/2020.12.13.20248143: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study protocols were approved by the University of Melbourne Human Research Ethics Committee (#2056689).
Consent: All subjects provided written informed consent in accordance with the Declaration of Helsinki.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Convalescent donors either had a PCR+ test during early infection or clear exposure to SARS-CoV-2, and were confirmed to have SARS-CoV-2 S- and RBD-specific antibodies via ELISA as previously reported (1). SARS-CoV-2suggested: NoneTo stain for S-expression, transduced cells were incubated with convalescent plasma (1:100 dilution) prior to staining with a secondary mouse anti-human IgG-APC antibody (1:200 dilution; clone HP6017, BioLegend). anti-human IgG-APCsuggested: NoneCorrelations between functional ADCC and ADP responses with cell-associated S-specific IgG and FcγR-binding S-specific antibodies were assessed using the non-parametric Spearman test. FcγR-binding S-specificsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cell lines: As target cells for the functional antibody assays, Ramos and A549 cells stably expressing full-length SARS-CoV-2 S and the reporter proteins mOrange2 or luciferase were generated by lentiviral transduction (Fig. S2A). Ramossuggested: NoneIIA1.6/FcR-γ/FcγRIIIa V158 cells were transfected with a NF-κB response element driven nanoluciferase (NanoLuc) reporter construct (pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] (Promega) by nucleofection (Amaxa Kit T, Lonza) and selected in the presence of 200 µg/ml hygromycin. IIA1.6/FcR-γ/FcγRIIIa V158suggested: NoneThe next day, COVID-19 convalescent and uninfected plasma were serially diluted and 50 µl aliquots transferred to the aspirated A549 S-orange cells and incubated at 37°C, 60 min, 5% CO2. A549suggested: NoneCell-based THP-1 association assay: To assess the capacity of THP-1 monocytes to associate with S-expressing target cells via Ab-FcγR interactions, an assay using THP-1 cells as effectors and Ramos S-orange cells as targets was performed. THP-1suggested: CLS Cat# 300356/p804_THP-1, RRID:CVCL_0006)Software and Algorithms Sentences Resources The data was analyzed using FlowJo 10.7.1 (see Fig. FlowJosuggested: (FlowJo, RRID:SCR_008520)These analyses were carried out in R: A language and environment for statistical computing version 4.0.2. Statistics: Statistical analyses were performed with Graphpad Prism 8. Graphpad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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