Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry
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Abstract
Vaccines are on the front line-of-defense against infectious diseases, ranging from threats we are familiar with, including polio, tuberculosis, or HIV, to novel and emerging threats such as SARS-CoV-2. Successful development of new protein-based vaccines requires sophisticated and efficient development of storage and formulation conditions. Here we demonstrate the combined power of 2bind’s sophisticated buffer matrix FORMOscreen ® and NanoTemper Technologies’ novel Prometheus Panta high-throughput Dynamic Light Scattering/Nano Differential Scanning Fluorimetry instrument. We show that this combination can comprehensively improve critical biophysical parameters for the HIV-1 vaccine BG505-SOSIP and find the optimal formulation condition with unmatched efficiency.
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SciScore for 10.1101/2020.11.30.403873: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This showcases one limitation of such a continuous variation binding assay: The hydrodynamic radii of the involved species must not deviate from each other with a factor >2 (or in other words, the molecular weights of the …
SciScore for 10.1101/2020.11.30.403873: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This showcases one limitation of such a continuous variation binding assay: The hydrodynamic radii of the involved species must not deviate from each other with a factor >2 (or in other words, the molecular weights of the involved species should not be more different than 5-fold) [9]. A control with a non-related antibody (The NIST monoclonal antibody (NISTmAb) reference material, RM 8671) [12] did not show signs of an interaction and the average hydrodynamic radius continuously changed from the BG505-SOSIP radius to that of the antibody (Figure 4C).
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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