Discovery of rhodomyrtone as a broad-spectrum antiviral inhibitor with anti-SARS-CoV-2 activity

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1. 

   ScreenIT

   Nov 17, 2020

   SciScore for 10.1101/2020.11.14.382770: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The cells were then washed with PBS twice, blocked by 5% BSA and incubated with mouse anti-HSV-1 gD monoclonal antibody (1:500, Santa Cruze Biotechnology) for 2 h, and stained with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Thermo Fisher Scientific) for 1 h at 4 °C.	anti-HSV-1 suggested: None anti-mouse suggested: (Thermo Fisher Scientific Cat# 88-7774-75, RRID:AB_476399)
   After washing 3 times with PBS, the cells were incubated with rabbit …

   SciScore for 10.1101/2020.11.14.382770: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The cells were then washed with PBS twice, blocked by 5% BSA and incubated with mouse anti-HSV-1 gD monoclonal antibody (1:500, Santa Cruze Biotechnology) for 2 h, and stained with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Thermo Fisher Scientific) for 1 h at 4 °C.	anti-HSV-1 suggested: None anti-mouse suggested: (Thermo Fisher Scientific Cat# 88-7774-75, RRID:AB_476399)
   After washing 3 times with PBS, the cells were incubated with rabbit anti-NXF1/TAP primary antibody (1:500, Cell Signaling Technology) and Alexa Fluor 594 anti-rabbit secondary antibody (1:500, Thermo Fisher Scientific), followed by staining the nucleus with DAPI.	anti-NXF1/TAP suggested: None anti-rabbit suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cells, viruses, and compounds: African green monkey kidney epithelial cells (Vero, ATCC) and HEK293 T cells (ATCC) were cultured in DMEM containing 10% fetal bovine serum (FBS, Gibco Invitrogen) at 37 °C, 5% CO2.	HEK293 suggested: None
   The fused proteins Aly/Ref-EGFP and NXF1/TAP-mCherry were transiently expressed in HEK293T cells by transfection with lipofectamine 2000.	HEK293T suggested: None
   Confocal assay: Vero cells in confocal dishes were infected with HSV-1/Patton/GFP (MOI=0.2), and then treated with RDT at 1 μM.	Vero suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
   Software and Algorithms
   Sentences	Resources
   Statistical analyses were conducted using Prism, version 8.0.	Prism suggested: (PRISM, RRID:SCR_005375)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. 

   Version published to 10.1101/2020.11.14.382770v1 on bioRxiv

   Nov 16, 2020