Biparatopic sybody constructs neutralize SARS-CoV-2 variants of concern and mitigate emergence of drug resistance

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   ScreenIT

   Nov 11, 2020

   SciScore for 10.1101/2020.11.10.376822: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Since the RBD-Fc construct was incompatible with our ELISA format due to the inclusion of Protein A to capture an α-myc antibody, ELISA was performed only for the RBD-vYFP (50 nM) and the ECD (25 nM) and later on with the S-2P (25 nM).	α-myc suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   100–250 mL of suspension-adapted Expi293 cells (Thermo) were transiently transfected using Expifectamine according to the manufacturer protocol (Thermo), and expression was continued for 4–5 days in a humidified environment at 37°C, 8% CO2.	Expi293 suggested: RRID:CVCL_D615)
   A second purified RBD …

   SciScore for 10.1101/2020.11.10.376822: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Since the RBD-Fc construct was incompatible with our ELISA format due to the inclusion of Protein A to capture an α-myc antibody, ELISA was performed only for the RBD-vYFP (50 nM) and the ECD (25 nM) and later on with the S-2P (25 nM).	α-myc suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   100–250 mL of suspension-adapted Expi293 cells (Thermo) were transiently transfected using Expifectamine according to the manufacturer protocol (Thermo), and expression was continued for 4–5 days in a humidified environment at 37°C, 8% CO2.	Expi293 suggested: RRID:CVCL_D615)
   A second purified RBD construct, consisting of SARS-CoV-2 residues Arg319—Phe541 fused to a murine IgG1 Fc domain (RBD-Fc) expressed in HEK293 cells, was purchased from Sino Biological (Catalogue number: 40592-V05H, 300 μg were ordered)	HEK293 suggested: None
   Subsequently, S protein-pseudotyped VSV*ΔG(Luc) was added to Vero E6 cells grown in 96-well plates (25’000 cells/well).	Vero E6 suggested: None
   Software and Algorithms
   Sentences	Resources
   Images were acquired in an automatic manner with SerialEM on a K2 summit detector (Gatan) in counting mode at ×49,407 magnification (1.012 Å pixel size) and a defocus range from −0.9 to −1.9 μm.	SerialEM suggested: (SerialEM, RRID:SCR_017293)
   A final non-uniform refinement in cryoSPARC further improved resolution down to 3.1 Å.	cryoSPARC suggested: (cryoSPARC, RRID:SCR_016501)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 37, 38 and 39. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2020.11.10.376822 on bioRxiv

   Nov 10, 2020