Composition of the immunoglobulin G glycome associates with the severity of COVID-19
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Abstract
A large variation in the severity of disease symptoms is one of the key open questions in COVID-19 pandemics. The fact that only a small subset of people infected with SARS-CoV-2 develop severe disease suggests that there have to be some predisposing factors, but biomarkers that reliably predict disease severity have not been found so far. Since overactivation of the immune system is implicated in a severe form of COVID-19 and the IgG glycosylation is known to be involved in the regulation of different immune processes, we evaluated the association of inter-individual variation in IgG N-glycome composition with the severity of COVID-19. The analysis of 166 severe and 167 mild cases from hospitals in Spain, Italy and Portugal revealed statistically significant differences in the composition of the IgG N-glycome. The most notable difference was the decrease in bisecting N -acetylglucosamine (GlcNAc) in severe patients from all three cohorts. IgG galactosylation was also lower in severe cases in all cohorts, but the difference in galactosylation was not statistically significant after correction for multiple testing. To our knowledge, this is the first study exploring IgG N-glycome variability in COVID-19 severity.
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SciScore for 10.1101/2020.10.17.20214205: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The study protocol conformed to the ethical guidelines of the 1975 Declaration of Helsinki and the study was approved by ethical committees of ASST Papa Giovanni XXIII° Hospital in Bergamo, Hospital del Mar in Barcelona and by the institutional ethics committee of Centro Hospitalar Universitário do Porto (CHUP), Centro Hospitalar de Vila Nova de Gaia/Espinho (CHVNG) and Hospital Beatriz Angelo (HBA), Loures, Lisbon.
Consent: All participants gave informed consent.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not …
SciScore for 10.1101/2020.10.17.20214205: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The study protocol conformed to the ethical guidelines of the 1975 Declaration of Helsinki and the study was approved by ethical committees of ASST Papa Giovanni XXIII° Hospital in Bergamo, Hospital del Mar in Barcelona and by the institutional ethics committee of Centro Hospitalar Universitário do Porto (CHUP), Centro Hospitalar de Vila Nova de Gaia/Espinho (CHVNG) and Hospital Beatriz Angelo (HBA), Loures, Lisbon.
Consent: All participants gave informed consent.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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