Safe and effective two-in-one replicon-and-VLP minispike vaccine for COVID-19

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1. 

   ScreenIT

   Oct 4, 2020

   SciScore for 10.1101/2020.10.02.324046: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animal experiments: Mouse immunization studies were carried out in the animal housing facility of the Paul-Ehrlich-Institute, in compliance with the regulations of German animal protection laws and authorized by the responsible state authority (V54-19c20/15-F107/1058). 11-19 weeks old female BALB/c mice received one or two intramuscular injections of 1×106 ffu of either VSVΔG-minispike-eGFP (VSV G), or VSVΔG (VSV G) “empty” dissolved in 30 μl PBS, or an equal volume of PBS alone, four weeks apart (prime or prime/boost).
   Cell Line Authentication	not …

   SciScore for 10.1101/2020.10.02.324046: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animal experiments: Mouse immunization studies were carried out in the animal housing facility of the Paul-Ehrlich-Institute, in compliance with the regulations of German animal protection laws and authorized by the responsible state authority (V54-19c20/15-F107/1058). 11-19 weeks old female BALB/c mice received one or two intramuscular injections of 1×106 ffu of either VSVΔG-minispike-eGFP (VSV G), or VSVΔG (VSV G) “empty” dissolved in 30 μl PBS, or an equal volume of PBS alone, four weeks apart (prime or prime/boost).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   BHK-G43 cells (Hanika et al., 2005), kindly provided by Georg Herrler, and BSR-MG-on cells (Finke et al., 2003) were maintained in GMEM media containing 10% fetal bovine serum, 0,5% Pen/Strep, 1% MEMs/NEAAs and 19,5mL tryptose phosphate broth (Thermo Fisher).	BHK-G43 suggested: None
   Microscopy: For live cell imaging, VeroE6 or HEK293T cells were seeded into poly-D-lysine (Millipore-Sigma)-coated multiwell plates one day prior to infection with VSV replicons at the indicated MOIs or plasmid transfection by lipofection, respectively.	HEK293T suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
   Virus and serum dilutions were incubated at 37°C for 20 min before 50 μl of VeroE6 cells were added to each well.	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Animal experiments: Mouse immunization studies were carried out in the animal housing facility of the Paul-Ehrlich-Institute, in compliance with the regulations of German animal protection laws and authorized by the responsible state authority (V54-19c20/15-F107/1058). 11-19 weeks old female BALB/c mice received one or two intramuscular injections of 1×106 ffu of either VSVΔG-minispike-eGFP (VSV G), or VSVΔG (VSV G) “empty” dissolved in 30 μl PBS, or an equal volume of PBS alone, four weeks apart (prime or prime/boost).	BALB/c suggested: None
   Software and Algorithms
   Sentences	Resources
   Construction of cDNAs: NCBI Reference Sequence NC_045512.2 of nCoV, Wuhan isolate 1, was used to synthesize human codon optimized cDNAs encoding full length HA-tagged spike (S-HA), and minispike (Thermo Fisher GeneArt).	Thermo Fisher GeneArt suggested: None
   Data representation and statistical analysis: Statistical analyses were performed using GraphPad Prism version 8.4.3.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2020.10.02.324046 on bioRxiv

   Oct 2, 2020