Two Color Single Molecule Sequencing on GenoCare™ 1600 Platform to Facilitate Clinical Applications

  1. Fang Chen
  2. Bin Liu
  3. Meirong Chen
  4. Zefei Jiang
  5. Zhiliang Zhou
  6. Ping Wu
  7. Meng Zhang
  8. Huan Jin
  9. Linsen Li
  10. Liuyan Lu
  11. Qi Wang
  12. Huan Shang
  13. Bing Xie
  14. Lei Liu
  15. Xia Lin
  16. Weiyue Chen
  17. Jianfeng Xu
  18. Ruitao Sun
  19. Guangming Wang
  20. Jiao Zheng
  21. Jifang Qi
  22. Bo Yang
  23. Dexia Chen
  24. Lidong Zeng
  25. Gailing Li
  26. Yan Li
  27. Hui Lv
  28. Nannan Zhao
  29. Bo Zhou
  30. Wen Wang
  31. Jinsen Cai
  32. Siyu Liu
  33. Weiwei Luo
  34. Juan Zhang
  35. Yanhua Zhang
  36. Yongyi Lu
  37. Jicai Fan
  38. Haitao Dan
  39. Xuesen He
  40. Lichun Liu
  41. Yan Feng
  42. Jianglin Chen
  43. Wei Huang
  44. Lei Sun
  45. Qin Yan

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Abstract

With the rapid development of precision medicine industry, DNA sequencing becomes increasingly important as a research and diagnosis tool. For clinical applications, medical professionals require a platform which is fast, easy to use, and presents clear information relevant to definitive diagnosis. We have developed a single molecule desktop sequencing platform, GenoCare™ 1600. Fast library preparation (without amplification) and simple instrument operation make it friendlier for clinical use. Here we presented sequencing data of E. coli sample from GenoCare™ 1600 with consensus accuracy reaches 99.99%. We also demonstrated sequencing of microbial mixtures and COVID-19 samples from throat swabs. Our data show accurate quantitation of microbial, sensitive identification of SARS-CoV-2 virus and detection of variants confirmed by Sanger sequencing.

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  1. ScreenIT

    SciScore for 10.1101/2020.09.28.20203455: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    No key resources detected.

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

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  2. Version published to 10.1101/2020.09.28.20203455v1 on medRxiv