Single-cell RNA Expression of SARS-CoV-2 Cell Entry Factors in Human Endometrium during Preconception

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Abstract

We investigated potential SARS-CoV-2 tropism in human endometrium by single-cell RNA-sequencing of viral entry-associated genes in healthy women. Percentages of endometrial cells expressing ACE2, TMPRSS2, CTSB , or CTSL were <2%, 12%, 80%, and 80%, respectively, with 0.7% of cells expressing all four genes. Our findings imply low efficiency of SARS-CoV-2 infection in the endometrium before embryo implantation, providing information to assess preconception risk in asymptomatic carriers.

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  1. SciScore for 10.1101/2020.09.14.296806: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Subject details: All procedures involving human endometrium were conducted in accordance with the Institutional Review Board (IRB) guidelines for Stanford University under the IRB code IRB-35448 and IVI Valencia, Spain under the IRB code 1603-IGX-016-CS.
    Consent: Informed written consent was obtained from each donor in her natural menstrual cycle (no hormone stimulation) before an endometrial biopsy was performed.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variableDe-identified human endometrium was obtained from women aged 18-34, with regular menstrual cycle (3-4 days every 28-30 days), BMI ranging 19-29 …