Small molecules inhibit SARS-COV-2 induced aberrant inflammation and viral replication in mice by targeting S100A8/A9-TLR4 axis

Article activity feed

1. 

   ScreenIT

   Sep 12, 2020

   SciScore for 10.1101/2020.09.09.288704: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All procedures of animal handling were approved by the Animal Care Committee of Peking University Health Science Center.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Male and female hACE2 mice on a C57BL/6J background were obtained from the Institute of Laboratory Animal Science, Peking Union Medical
   Cell Line Authentication	Contamination: Cells were negative for mycoplasma.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After blocking non-specific Fc-mediated interactions with CD16/CD32 antibodies (14-0161-82, eBioscience, USA), single-cell suspensions were stained with fluorophore-conjugated anti-mouse …

   SciScore for 10.1101/2020.09.09.288704: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All procedures of animal handling were approved by the Animal Care Committee of Peking University Health Science Center.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Male and female hACE2 mice on a C57BL/6J background were obtained from the Institute of Laboratory Animal Science, Peking Union Medical
   Cell Line Authentication	Contamination: Cells were negative for mycoplasma.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After blocking non-specific Fc-mediated interactions with CD16/CD32 antibodies (14-0161-82, eBioscience, USA), single-cell suspensions were stained with fluorophore-conjugated anti-mouse antibodies at 4°C for 30min.	CD16/CD32 suggested: (Thermo Fisher Scientific Cat# 14-0161-82, RRID:AB_467133) anti-mouse suggested: None 30min suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   VSV, EMCV, HSV-1 were propagated in Vero cells followed by 3 cycles of freezing and thawing, then the large debrises were spun down and the supernatants were used as a stock solution.	Vero suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
   The allantoic fluid was collected and titrated to determine the 50% tissue culture infection dose (TCID50) in A549 cells and the median lethal dose (LD50) in mice following the Reed-Muench method.	A549 suggested: None
   Cell culture: Raw 274.7 cells, 17CL-1 cells, MDCK cells and Vero cells were kept in our lab.	MDCK suggested: CLS Cat# 602280/p823_MDCK_(NBL-2, RRID:CVCL_0422)
   Raw 274.7 cells were cultured in 1640 medium (Gibco) supplemented with 10% FBS, 100 U/mL Penicillin-Streptomycin.	Raw 274.7 suggested: None
   Raw 264.7 cells were seeded on 6-well plates with 106 cells/mL.	Raw 264.7 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Irf3/7 double knockout mice on a C57BL/6J background was a gift from Pro. Zhengfan Jiang.	C57BL/6J suggested: RRID:IMSR_JAX:000664)
   Before being inoculated intranasally with reagents and viruses, hACE2 mice were intraperitoneally anaesthetized by 2.5% avertin with 0.02 mL/g body weight; WT C57BL/6J mice, interferon-α receptor gene knockout mice (Ifnar-/-), Elf4-/- mice, Ifnar-/- mice and Mavs-/- mice were anaesthetized by isoflurane; rhesus macaques were anaesthetized with 10 mg/kg ketamine hydrochloride.	Elf4-/- suggested: None Mavs-/- suggested: None
   Resatorvid rescue assay/Azeliragon rescue assay: Ifnar-/- mice were inoculated intraperitoneally with MHV-A59.	Ifnar-/- suggested: None
   Software and Algorithms
   Sentences	Resources
   The raw images were transformed into raw reads by base calling using CASAVA (http://www.illumina.com/support/documentation.ilmn).	CASAVA suggested: (CASAVA, RRID:SCR_001802)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

   ---

   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 22. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Read the original source
2. 

   Version published to 10.1101/2020.09.09.288704v1 on bioRxiv

   Sep 9, 2020