Coronacept – a potent immunoadhesin against SARS-CoV-2
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Abstract
Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Computational analysis of mammalian ACE2 orthologues suggests various residues at the interface with the viral receptor binding domain that could facilitate tighter interaction compared to the human-ACE2. Introducing several mutations to the human-ACE2 resulted with significantly augmented affinity to the viral spike complex. This modified human-ACE2 fused to an Fc portion of an antibody makes a potent immunoadhesin that effectively targets SARS-CoV-2.
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SciScore for 10.1101/2020.08.12.247940: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Lentiviral particles production and Neutralization: Lentiviruses expressing S-Covid19 spikes were produced by transfecting HEK293T cells with Luciferase-pLenti6, Δ19 S_covid-pCMV3 and ΔR89 Ψ vectors at 1:1:1 ratio, using Lipofectamine 2000 (Thermo Fisher). HEK293Tsuggested: NoneSoftware and Algorithms Sentences Resources Atomistic modeling: Orthologous sequences of ACE2 were collected by using a protein BLAST 33 search of the human-ACE2 … SciScore for 10.1101/2020.08.12.247940: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Lentiviral particles production and Neutralization: Lentiviruses expressing S-Covid19 spikes were produced by transfecting HEK293T cells with Luciferase-pLenti6, Δ19 S_covid-pCMV3 and ΔR89 Ψ vectors at 1:1:1 ratio, using Lipofectamine 2000 (Thermo Fisher). HEK293Tsuggested: NoneSoftware and Algorithms Sentences Resources Atomistic modeling: Orthologous sequences of ACE2 were collected by using a protein BLAST 33 search of the human-ACE2 sequence at GenBank and filtering the results to mammalian origin, and to sequences with greater than 80% identity to human-ACE2. BLASTsuggested: (BLASTX, RRID:SCR_001653)Sequences were aligned using MUSCLE 34. MUSCLEsuggested: (MUSCLE, RRID:SCR_011812)Curve fitting were performed using GraphPad Prism. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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