A valid protective immune response elicited in rhesus macaques by an inactivated vaccine is capable of defending against SARS-CoV-2 infection

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1. 

   ScreenIT

   Aug 11, 2020

   SciScore for 10.1101/2020.08.04.235747: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Virus and cells: The SARS-CoV-2 virus used was isolated from the respiratory secretions of an adult male patient at Yunnan Hospital of Infectious Diseases in Kunming in January 2020.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   , anti-N antibody (Snio Biological) and horseradish peroxidase (HRP)-labeled secondary antibody (Abcam, MA, USA) to visualize the proteins according to the standard protocol of the enhanced chemiluminescence (ECL) reagent	anti-N suggested: None
   Electron microscopy: Purified inactivated …

   SciScore for 10.1101/2020.08.04.235747: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Virus and cells: The SARS-CoV-2 virus used was isolated from the respiratory secretions of an adult male patient at Yunnan Hospital of Infectious Diseases in Kunming in January 2020.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   , anti-N antibody (Snio Biological) and horseradish peroxidase (HRP)-labeled secondary antibody (Abcam, MA, USA) to visualize the proteins according to the standard protocol of the enhanced chemiluminescence (ECL) reagent	anti-N suggested: None
   Electron microscopy: Purified inactivated SARS-CoV-2 preparations were coincubated with convalescent serum, a monoclonal antibody (mAb) against S (mAb-S) or N (mAb-N) (Solarbio, Beijing, China) at 37°C for 24 h, stained with 1% phosphotungstic acid and observed by transmission electron microscopy (Hitachi, Kyoto, Japan).	mAb-N suggested: None
   Anti-CD3, anti-CD20 and anti-CD16 antibodies were added to the PBMCs.	Anti-CD3 , suggested: None anti-CD20 suggested: None anti-CD16 suggested: None
   The PBMCs were coincubated with an anti-CD4 antibody (BD) at RT for 30 min.	anti-CD4 suggested: None
   After incubating with the red blood cell lysis and membrane permeabilization reagents and washing with PBS twice, the cells were stained with anti-FOXP3, anti-IL-4, anti-IFN-γ and anti-IL-17A antibodies for 30 min at RT and then washed once.	anti-FOXP3 suggested: None anti-IL-4 suggested: None anti-IFN-γ suggested: (Bio-Rad Cat# M6000007NY, RRID:AB_2784537) anti-IL-17A suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Vero cells were cultured in DMEM (Corning, NY, USA) containing 5% fetal bovine serum (FCS; HyClone, Logan, USA).	Vero suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
   Software and Algorithms
   Sentences	Resources
   The transferred membrane was blocked in 5% bovine serum albumin (BSA)-Tris-buffered saline/Tween 20 (Tris-HCl, 100 mM, pH 7.5; NaCl, 0.9%; and Tween-20, 0.2%) and treated with convalescent serum, anti-S (Snio Biological, Beijing,	Snio Biological suggested: None
   GraphPad Prism software (San Diego, CA, USA) and STATA (Version 15.0; STATA Corp., College Station, TX, USA) were used for statistical analyses.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) STATA suggested: (Stata, RRID:SCR_012763)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2020.08.04.235747v1 on bioRxiv

   Aug 4, 2020