Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients

  1. John A. Lednicky
  2. Michael Lauzardo
  3. Z. Hugh Fan
  4. Antarpreet Jutla
  5. Trevor B. Tilly
  6. Mayank Gangwar
  7. Moiz Usmani
  8. Sripriya Nannu Shankar
  9. Karim Mohamed
  10. Arantza Eiguren-Fernandez
  11. Caroline J. Stephenson
  12. Md. Mahbubul Alam
  13. Maha A. Elbadry
  14. Julia C. Loeb
  15. Kuttichantran Subramaniam
  16. Thomas B. Waltzek
  17. Kartikeya Cherabuddi
  18. J. Glenn Morris
  19. Chang-Yu Wu

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  1. ScreenIT

    SciScore for 10.1101/2020.08.03.20167395: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: An abbreviated summary of methods is provided below: Institutional Review Board (IRB) approval and patients: The study protocol was approved by the UF IRB (study IRB202002102).
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    23 Next-generation sequencing the genome of SARS-CoV-2 isolated from NP swab: The vRNA extracted from virions in spent Vero E6 cell culture medium served as a template to generate a cDNA library using a NEBNext Ultra II RNA Library Prep kit (New England Biolabs, Inc.)
    Vero E6
    suggested: RRID:CVCL_XD71)
    Software and Algorithms
    SentencesResources
    Following the removal of host sequences (Chlorocebus sabaeus; GenBank assembly accession number GCA_000409795.2) using Kraken 2,29 de novo assembly of paired-end reads was performed in SPAdes v3.13.0 with default parameters.
    Kraken
    suggested: (Kraken, RRID:SCR_005484)
    SPAdes
    suggested: (SPAdes, RRID:SCR_000131)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1016/j.ijid.2020.09.025
  3. Version published to 10.1101/2020.08.03.20167395v1 on medRxiv