Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients
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Abstract
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SciScore for 10.1101/2020.08.03.20167395: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: An abbreviated summary of methods is provided below: Institutional Review Board (IRB) approval and patients: The study protocol was approved by the UF IRB (study IRB202002102). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 23 Next-generation sequencing the genome of SARS-CoV-2 isolated from NP swab: The vRNA extracted from virions in spent Vero E6 cell culture medium served as a template to generate a cDNA library using a NEBNext Ultra II RNA Library Prep kit (New England Biolabs, Inc.) V…SciScore for 10.1101/2020.08.03.20167395: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: An abbreviated summary of methods is provided below: Institutional Review Board (IRB) approval and patients: The study protocol was approved by the UF IRB (study IRB202002102). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 23 Next-generation sequencing the genome of SARS-CoV-2 isolated from NP swab: The vRNA extracted from virions in spent Vero E6 cell culture medium served as a template to generate a cDNA library using a NEBNext Ultra II RNA Library Prep kit (New England Biolabs, Inc.) Vero E6suggested: RRID:CVCL_XD71)Software and Algorithms Sentences Resources Following the removal of host sequences (Chlorocebus sabaeus; GenBank assembly accession number GCA_000409795.2) using Kraken 2,29 de novo assembly of paired-end reads was performed in SPAdes v3.13.0 with default parameters. Krakensuggested: (Kraken, RRID:SCR_005484)SPAdessuggested: (SPAdes, RRID:SCR_000131)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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