CoVaccine HT™ adjuvant potentiates robust immune responses to recombinant SARS-CoV-2 Spike S1 immunisation

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1. 

   ScreenIT

   Jul 26, 2020

   SciScore for 10.1101/2020.07.24.220715: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	2.1 Vaccination and Serum Collection: BALB/c mice (7-8 weeks of age, male and female) were immunised twice, three weeks apart, intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants, or adjuvant alone, using an insulin syringe with a 29-gauge needle.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution.	anti-mouse suggested…

   SciScore for 10.1101/2020.07.24.220715: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	2.1 Vaccination and Serum Collection: BALB/c mice (7-8 weeks of age, male and female) were immunised twice, three weeks apart, intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants, or adjuvant alone, using an insulin syringe with a 29-gauge needle.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution.	anti-mouse suggested: None
   The tests were set up in duplicates, and the costimulatory anti-CD28 antibody (0.1 μg/mL) was added to the cells during the incubation.	anti-CD28 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   The serum-virus mixtures were added to a monolayer of confluent Vero E6 cells and incubated for 1 hour at 37°C in 5% CO2.	Vero E6 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   2.1 Vaccination and Serum Collection: BALB/c mice (7-8 weeks of age, male and female) were immunised twice, three weeks apart, intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants, or adjuvant alone, using an insulin syringe with a 29-gauge needle.	BALB/c suggested: None
   Software and Algorithms
   Sentences	Resources
   Cut-offs were generated by determining the mean MFI values plus three standard deviations as determined by Microsoft Office Excel program	Microsoft Office Excel suggested: (Microsoft Excel, RRID:SCR_016137)
   Graphical representation of the data was done using Prism, Graphpad Software (San Diego, CA).	Prism suggested: (PRISM, RRID:SCR_005375) Graphpad suggested: (GraphPad, RRID:SCR_000306)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2020.07.24.220715 on bioRxiv

   Jul 26, 2020