High Community SARS-CoV-2 Antibody Seroprevalence in a Ski Resort Community, Blaine County, Idaho, US. Preliminary Results
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Abstract
Community-level seroprevalence surveys are needed to determine the proportion of the population with previous SARS-CoV-2 infection, a necessary component of COVID-19 disease surveillance. In May, 2020, we conducted a cross-sectional seroprevalence study of IgG antibodies for nucleocapsid of SARS-CoV-2 among the residents of Blaine County, Idaho, a ski resort community with high COVID-19 attack rates in late March and Early April (2.9% for ages 18 and older). Participants were selected from volunteers who registered via a secure web link, using prestratification weighting to the population distribution by age and gender within each ZIP Code. Participants completed a survey reporting their demographics and symptoms; 88% of volunteers who were invited to participate completed data collection survey and had 10 ml of blood drawn. Serology was completed via the Abbott Architect SARS-CoV-2 IgG immunoassay. Primary analyses estimated seroprevalence and 95% credible intervals (CI) using a hierarchical Bayesian framework to account for diagnostic uncertainty. Stratified models were run by age, sex, ZIP Code, ethnicity, employment status, and a priori participant-reported COVID-19 status. Sensitivity analyses to estimate seroprevalence included base models with post-stratification for ethnicity, age, and sex, with or without adjustment for multi-participant households. IgG antibodies to the virus that causes COVID-19 were found among 22.7% (95% CI: 20.1%, 25.5%) of residents of Blaine County. Higher levels of antibodies were found among residents of the City of Ketchum 34.8% (95% CI 29.3%, 40.5%), compared to Hailey 16.8% (95%CI 13.7%, 20.3%) and Sun Valley 19.4% (95% 11.8%, 28.4%). People who self-identified as not believing they had COVID-19 had the lowest prevalence 4.8% (95% CI 2.3%, 8.2%). The range of seroprevalence after correction for potential selection bias was 21.9% to 24.2%. This study suggests more than 80% of SARS-CoV-2 infections were not reported. Although Blaine County had high levels of SARS-CoV-2 infection, the community is not yet near the herd immunity threshold.
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SciScore for 10.1101/2020.07.19.20157198: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Invitations to participate were emailed to sampled volunteers with a link to an electronic consent statement and questionnaire on demographic and symptom history.
IRB: The study was approved by the Fred Hutchinson Cancer Research Center (Fred Hutch) Institutional Review Board.Randomization Volunteers were selected randomly for participation after stratification by ZIP Code, and by age and gender within ZIP Code. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Plasma was separated from cellular fraction by centrifugation at 1200 x g for 15 minutes, … SciScore for 10.1101/2020.07.19.20157198: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Invitations to participate were emailed to sampled volunteers with a link to an electronic consent statement and questionnaire on demographic and symptom history.
IRB: The study was approved by the Fred Hutchinson Cancer Research Center (Fred Hutch) Institutional Review Board.Randomization Volunteers were selected randomly for participation after stratification by ZIP Code, and by age and gender within ZIP Code. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Plasma was separated from cellular fraction by centrifugation at 1200 x g for 15 minutes, transferred into cryovials, and aliquots were sent to the University of Washington for testing via the Abbott Architect SARS-CoV-2 IgG chemiluminescent microparticle immunoassay, according to manufacturer protocols. Abbott Architectsuggested: (Abbott ARCHITECT i1000sr System, RRID:SCR_019328)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
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SciScore for 10.1101/2020.07.19.20157198: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Invitations to participate were emailed to sampled volunteers with a link to an electronic consent statement and questionnaire on demographic and symptom history. Randomization Volunteers were selected randomly for participation after stratification by ZIP Code, and by age and gender within ZIP Code. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Of 917 participants, 208 (22.7%, 95% CI: 20.0%, 25.5%) had IgG antibodies. had IgGsuggested: NoneSoftware and Algorithms Sentences Resources Specimen Collection and … SciScore for 10.1101/2020.07.19.20157198: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Invitations to participate were emailed to sampled volunteers with a link to an electronic consent statement and questionnaire on demographic and symptom history. Randomization Volunteers were selected randomly for participation after stratification by ZIP Code, and by age and gender within ZIP Code. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Of 917 participants, 208 (22.7%, 95% CI: 20.0%, 25.5%) had IgG antibodies. had IgGsuggested: NoneSoftware and Algorithms Sentences Resources Specimen Collection and Antibody testing Blood was collected from May 419, 2020 using standard protocols into 10 cc vials with acid citrate dextrose additive to prevent clotting and shipped overnight to the Fred Hutch laboratory Plasma was separated from cellular fraction by centrifugation at 1200 x g for 15 minutes, transferred into cryovials, and aliquots were sent to the University of Washington for testing via the Abbott Architect SARS-CoV-2 IgG chemiluminescent microparticle immunoassay, accordin to manufacturer protocols. Abbott Architectsuggested: (Abbott ARCHITECT i1000sr System, SCR_018371)Data from additional tools added to each annotation on a weekly basis.
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