Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

Article activity feed

1. Version published to 10.1016/j.jviromet.2020.114048

   Mar 1, 2021
2. 

   ScreenIT

   Jul 21, 2020

   SciScore for 10.1101/2020.07.16.20155168: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   Samples were run on an Aria qPCR Cycler (Agilent) and results analysed using the Agilent AriaMX 1.5 software.	Agilent AriaMX suggested: (Agilent AriaMx Real-time PCR System, RRID:SCR_019469)
   Preparation of 10% (w/v) Chelex® 100 Resin: 10% (w/v) Chelex® 100 Resin was made up by resuspending Chelex® 100 Resin (200-400 mesh) (Bio-Rad Laboratories, catalogue number #142-1253) in Milli-Q® water at 10% (w/v).	Bio-Rad Laboratories suggested: (Bio-Rad Laboratories, RRID:SCR_008426)

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   Results …

   SciScore for 10.1101/2020.07.16.20155168: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   Samples were run on an Aria qPCR Cycler (Agilent) and results analysed using the Agilent AriaMX 1.5 software.	Agilent AriaMX suggested: (Agilent AriaMx Real-time PCR System, RRID:SCR_019469)
   Preparation of 10% (w/v) Chelex® 100 Resin: 10% (w/v) Chelex® 100 Resin was made up by resuspending Chelex® 100 Resin (200-400 mesh) (Bio-Rad Laboratories, catalogue number #142-1253) in Milli-Q® water at 10% (w/v).	Bio-Rad Laboratories suggested: (Bio-Rad Laboratories, RRID:SCR_008426)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.07.16.20155168 on medRxiv

   Jul 21, 2020