SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damage and constitutes an antiviral target

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1. 

   ScreenIT

   Jul 2, 2020

   SciScore for 10.1101/2020.06.27.174953: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All animal procedures were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, under protocols approved and strictly followed by the Institutional Animal Care and Use Committees (IACUC)
   Randomization	All image data shown are representative of at least three randomly selected fields.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Male C57BL/6 mice were obtained from Shanghai SLAC Laboratory Animal Co., Ltd.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Blots were probed with antibodies against HA-Tag (C29F4) (3724, CST, USA), GSDMD …

   SciScore for 10.1101/2020.06.27.174953: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All animal procedures were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, under protocols approved and strictly followed by the Institutional Animal Care and Use Committees (IACUC)
   Randomization	All image data shown are representative of at least three randomly selected fields.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Male C57BL/6 mice were obtained from Shanghai SLAC Laboratory Animal Co., Ltd.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Blots were probed with antibodies against HA-Tag (C29F4) (3724, CST, USA), GSDMD (L60) (93709, CST, USA),	HA-Tag suggested: (Cell Signaling Technology Cat# 3724, RRID:AB_1549585)
   Experimental Models: Cell Lines
   Sentences	Resources
   Cell culture and treatment: HEK293, MCF-7, Caco2, Vero E6, HeLa, HepG2, SH-SY5Y cells were grown in 90% DMEM basal medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA), 2 mM L-glutamine and 100 units /mL penicillin/streptomycin (Gibco, USA).	MCF-7 suggested: None Caco2 suggested: None Vero E6 suggested: RRID:CVCL_XD71) HeLa suggested: None HepG2 suggested: None SH-SY5Y suggested: None
   1% NEAA (Gibco, USA) was added in above medium for A498 cells culture.	A498 suggested: NCI-DTP Cat# A498, RRID:CVCL_1056)
   Besides, HCT116 and HT-29 cells were grown in McCoy’s 5A basal medium (Gibco, USA), PC3 and A549 cells were grown in RPMI-1640 basal medium (Hyclone, USA) and CHO cells were grown in DMEM/F-12 basal medium (Gibco, USA) supplemented as above. 16HBE cells were grown in KM (ScienCell, USA) medium.	HCT116 suggested: None HT-29 suggested: None PC3 suggested: None A549 suggested: None CHO suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Male C57BL/6 mice were obtained from Shanghai SLAC Laboratory Animal Co., Ltd.	C57BL/6 suggested: None
   Software and Algorithms
   Sentences	Resources
   The percentages of differently labeled cells were calculated by FlowJo 7.6.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   The MS raw data were analyzed with MaxQuant (http://maxquant.org/,	MaxQuant suggested: (MaxQuant, RRID:SCR_014485)
   The currents were digitized using pClamp 10.2 software (Molecular Devices, US).	pClamp suggested: (pClamp, RRID:SCR_011323)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   One limitation of our study is the lack of evidence in the context of SARS-CoV-2 infection in vivo, which can technically be achieved by generating of SARS-CoV-2 without 2-E using reverse genetics. However, the 2-E deleted SARS-CoV-2 may replicate more effectively and thus this experiment was not conducted. Although the in vivo antiviral activity of the newly identified channel inhibitors needs further studies, their potent antiviral activity in vitro and excellent protection effects against ARDS-like damage in vivo shed light on the drug development of 2-E channel inhibitors. Given that 2-E can function as ion channels in the viral membranes, similar to how they function in host cells, we propose that 2-E channels may represent a new class of dualfunction targets against SARS-CoV-2.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 21. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. 

   Version published to 10.1101/2020.06.27.174953 on bioRxiv

   Jun 29, 2020