Engineered human mesenchymal stem cells as new vaccine platform for COVID-19
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Abstract
Recently, there are several routes for COVID-19 vaccine research, yet their weaknesses lie in low efficiency, tolerability, immune adaptability and safety. We describe a new approach to COVID-19 based on engineered human mesenchymal stem cells(hu-MSC), which is like a small protein antigen response device, but will be gradually cleared and degraded by body’s immune system among recognization process. The antibody response results show that this is effective and fast. Furthermore, after several antibody response tests, we obtained an injection of a set of cocktail-like modified human mesenchymal stem cell line. This strategy opened a new avenue for vaccine design against COVID-19.
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SciScore for 10.1101/2020.06.20.163030: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Animal works were approved by ethical committee of Shenyang University of Chemical Technology. Randomization Then, the mice were randomly divided into two groups, according to the two injection ways (intravenous and subculaneous). Blinding not detected. Power Analysis not detected. Sex as a biological variable Animal: All mice in our experiment were provided by Changsheng Animal Research Institute in Liaoning Province, and Male and female C57BL/6 mice (4-8 weeks old) were used for immune injection. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources We assessed the ability of mice to produce antibodies against nucleocapsid protein by … SciScore for 10.1101/2020.06.20.163030: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Animal works were approved by ethical committee of Shenyang University of Chemical Technology. Randomization Then, the mice were randomly divided into two groups, according to the two injection ways (intravenous and subculaneous). Blinding not detected. Power Analysis not detected. Sex as a biological variable Animal: All mice in our experiment were provided by Changsheng Animal Research Institute in Liaoning Province, and Male and female C57BL/6 mice (4-8 weeks old) were used for immune injection. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources We assessed the ability of mice to produce antibodies against nucleocapsid protein by ELISA analysis. antibodies against nucleocapsid protein by ELISA analysis.suggested: NoneExperimental Models: Cell Lines Sentences Resources Hu-MSC cell isolation: The fresh umbilical cord tissue was cut into some pieces and isolated enzymatically in an incubation solution of 10mL Trypsin-EDTA (Cat: Hu-MSCsuggested: NoneExperimental Models: Organisms/Strains Sentences Resources Generation of vectored antigen protein by eukaryotic expressing system: Refferring to the SARS-COV-2 virus genome sequence from Wuhan-Hu-1 annotated in Genebank (locus:NC_045512.2 or MN908947)[23], we synthesized the spike gene, membrane gene and nucleocapsid gene in gene synthesis company (GENEWIZ, china). MN908947suggested: NoneEngineered hu-MSC injection and in vivo immunization: An equal amount of male and female C57BL/6 mice, aged from six to eight weeks, were taken. C57BL/6suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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