Rapid Inactivation of SARS-CoV-2 by Silicon Nitride, Copper, and Aluminum Nitride
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Abstract
Introduction
Viral disease spread by contaminated commonly touched surfaces is a global concern. Silicon nitride, an industrial ceramic that is also used as an implant in spine surgery, has known antibacterial activity. The mechanism of antibacterial action relates to the hydrolytic release of surface disinfectants. It is hypothesized that silicon nitride can also inactivate the coronavirus SARS-CoV-2.
Methods
SARS-CoV-2 virions were exposed to 15 wt.% aqueous suspensions of silicon nitride, aluminum nitride, and copper particles. The virus was titrated by the TCD 50 method using VeroE6/TMPRSS2 cells, while viral RNA was evaluated by real-time RT-PCR. Immunostaining and Raman spectroscopy were used as additional probes to investigate the cellular responses to virions exposed to the respective materials.
Results
All three tested materials showed >99% viral inactivation at one and ten minutes of exposure. Degradation of viral RNA was also observed with all materials. Immunofluorescence testing showed that silicon nitride-treated virus failed to infect VeroE6/TMPRSS2 cells without damaging them. In contrast, the copper-treated virus suspension severely damaged the cells due to copper ion toxicity. Raman spectroscopy indicated differential biochemical cellular changes due to infection and metal toxicity for two of the three materials tested.
Conclusions
Silicon nitride successfully inactivated the SARS-CoV-2 in this study. The mechanism of action was the hydrolysis-mediated surface release of nitrogen-containing disinfectants. Both aluminum nitride and copper were also effective in the inactivation of the virus. However, while the former compound affected the cells, the latter compound had a cytopathic effect. Further studies are needed to validate these findings and investigate whether silicon nitride can be incorporated into personal protective equipment and commonly touched surfaces, as a strategy to discourage viral persistence and disease spread.
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SciScore for 10.1101/2020.06.19.159970: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The cells were blocked with 2% skim milk in TBS for 60 min at RT and stained with anti-SARS Coronavirus envelope (Rabbit) antibody (Dilution =1:100) (ProSci Inc., Poway, CA, USA) for 60 min at RT. anti-SARSsuggested: NoneExperimental Models: Cell Lines Sentences Resources Immunofluorescence: Vero E6/TMPRSS2 cells on cover glass were inoculated with 200 μL of virus supernatant. Vero E6/TMPRSS2suggested: NoneSoftware and Algorithms Sentences Resources Statistical … SciScore for 10.1101/2020.06.19.159970: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The cells were blocked with 2% skim milk in TBS for 60 min at RT and stained with anti-SARS Coronavirus envelope (Rabbit) antibody (Dilution =1:100) (ProSci Inc., Poway, CA, USA) for 60 min at RT. anti-SARSsuggested: NoneExperimental Models: Cell Lines Sentences Resources Immunofluorescence: Vero E6/TMPRSS2 cells on cover glass were inoculated with 200 μL of virus supernatant. Vero E6/TMPRSS2suggested: NoneSoftware and Algorithms Sentences Resources Statistical Analysis: The Student’s t-test determined statistical significance for n=3 and at a p-value of 0.01 using Prism software (GraphPad, San Diego, CA USA). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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