Epidemiological description and analysis of RdRp, E and N genes dynamic by RT-PCR of SARS-CoV-2 in Moroccan population: Experience of the National Reference Laboratory (LNR)-UM6SS
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Abstract
The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a new infectious disease that first emerged in Hubei province, China, in December 2019. On 2 March 2020, the Moroccan Ministry of Health confirmed the first COVID-19 case in Morocco. The new virus SARS-CoV-2 was identified in the sample of a Moroccan expatriate residing in Italy. Without a therapeutic vaccine or specific antiviral drugs, early detection and isolation become essential against novel Coronavirus.
This study aims to analyze the epidemiological profile of the SARS-CoV-2 in Moroccan cases and to investigate the dynamic of RdRp, N , and E genes in patients from diagnosis until the recovery.
Among 859 COVID-19 RT-PCR tests realized for 376 patients, 187 cases had positive results COVID-19. 4% were positive with the 3 genes RdRp, N , and E , 40 % with N and E genes, 3% with RdRp and N genes, 31% with only the RdRp gene and 22% cases are positives with N gene. The analysis of the Covid-19 genes ( RdRp, N , and E ) dynamic reveal that more than 6% stay positive with detection of the N and E gene, and 14% with the N gene after 12 days of treatment.
The median period from positive to the first negative Covid-19 RT-PCR tests was 6.8±2.24 days for 44% cases, 14.31± 2.4 days for 30%, and 22.67 ± 1.21 days for 4%.
This a first description of the Moroccan COVID-19 cases and the analysis of the dynamic of the RdRp, N , and E genes. The analysis of our population can help to improve in the care of patients.
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SciScore for 10.1101/2020.06.18.20135137: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The mean age was 46.82 ± 20.54 years old, comprising 55.76 % men and 44.24% women (table 2). Table 2: Resources
Software and Algorithms Sentences Resources The RT-PCR assays were performed on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.). Bio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)All statistical analyses were performed using STATA software, version 11.0. 3. Results: 3.1. STATAsuggested: (Stata, RRID:SCR_012763)Results from OddPub: We did not detect open data. We also did not detect open code. …
SciScore for 10.1101/2020.06.18.20135137: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The mean age was 46.82 ± 20.54 years old, comprising 55.76 % men and 44.24% women (table 2). Table 2: Resources
Software and Algorithms Sentences Resources The RT-PCR assays were performed on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.). Bio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)All statistical analyses were performed using STATA software, version 11.0. 3. Results: 3.1. STATAsuggested: (Stata, RRID:SCR_012763)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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