Holder Pasteurization Inactivates SARS-CoV-2 in Human Breast Milk

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1. 

   ScreenIT

   Jun 18, 2020

   SciScore for 10.1101/2020.06.17.155689: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Milk samples: Milk samples were obtained from five healthy human donors after ethical approval by the ethics commission of Hanover Medical School, Hanover, Germany and the ethics committee of Ulm University. Consent: All mothers provided written informed consent for the collection of samples and subsequent analysis.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus was propagated by inoculation of 70% confluent Vero E6 cells in 75 cm2 cell culture flasks with 100 μl SARS-CoV-2 isolates …

   SciScore for 10.1101/2020.06.17.155689: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Milk samples: Milk samples were obtained from five healthy human donors after ethical approval by the ethics commission of Hanover Medical School, Hanover, Germany and the ethics committee of Ulm University. Consent: All mothers provided written informed consent for the collection of samples and subsequent analysis.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus was propagated by inoculation of 70% confluent Vero E6 cells in 75 cm2 cell culture flasks with 100 μl SARS-CoV-2 isolates in 3.5 ml serum-free medium containing 1 μg/mL trypsin.	Vero E6 suggested: None
   TCID50 endpoint titration: To determine the tissue culture infectious dose 50 (TCID50), virus stocks or samples were serially diluted and used to inoculate Vero E6 or Caco-2 cells.	Caco-2 suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2020.06.17.155689v1 on bioRxiv

   Jun 17, 2020