Oral drug repositioning candidates and synergistic remdesivir combinations for the prophylaxis and treatment of COVID-19

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1. 

   ScreenIT

   Jun 17, 2020

   SciScore for 10.1101/2020.06.16.153403: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus generation: Vero E6 cells (ATCC CRL-1586) were plated in a T225 flask with complete DMEM (Corning 15-013-CV) containing 10% FBS, 1×PenStrep (Corning 20-002-CL), 2 mM L-Glutamine (Corning 25-005-CL) overnight at 37 □ 5% CO2.	Vero E6 suggested: None
   HeLa-ACE2 stable cell line: HeLa-ACE2 cells were generated through transduction of human ACE2 lentivirus.	HeLa-ACE2 suggested: None
   The lentivirus was created by co-transfection of HEK293T …

   SciScore for 10.1101/2020.06.16.153403: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus generation: Vero E6 cells (ATCC CRL-1586) were plated in a T225 flask with complete DMEM (Corning 15-013-CV) containing 10% FBS, 1×PenStrep (Corning 20-002-CL), 2 mM L-Glutamine (Corning 25-005-CL) overnight at 37 □ 5% CO2.	Vero E6 suggested: None
   HeLa-ACE2 stable cell line: HeLa-ACE2 cells were generated through transduction of human ACE2 lentivirus.	HeLa-ACE2 suggested: None
   The lentivirus was created by co-transfection of HEK293T cells with pBOB-hACE2 construct and lentiviral packaging plasmids pMDL	HEK293T suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
   Supernatant was collected 48 h after transfection then used to transduce pre-seeded HeLa cells.	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   Software and Algorithms
   Sentences	Resources
   (Molecular Devices) with a 10× objective, and total live cells per well quantified in the acquired images using the Live Dead Application Module (MetaXpress).	MetaXpress suggested: (MetaXpress, RRID:SCR_016654)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. 

   Version published to 10.1101/2020.06.16.153403 on bioRxiv

   Jun 16, 2020