Bepridil is potent against SARS-CoV-2 In Vitro

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1. 

   ScreenIT

   Jun 9, 2020

   SciScore for 10.1101/2020.05.23.112235: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   SARS-CoV-2 inhibition by a cell-based assay: A slightly modified standard live virus-based microneutralization (MN) assay was used as previously described(40-42) to rapidly evaluate the drug efficacy against SARS-CoV-2 infection in Vero E6 and A549 cell cultures.	Vero E6 suggested: None
   The toxicity to the treated cells was assessed by observing floating cells and altered morphology of adhered Vero E6 and A549 cells in wells under the …

   SciScore for 10.1101/2020.05.23.112235: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   SARS-CoV-2 inhibition by a cell-based assay: A slightly modified standard live virus-based microneutralization (MN) assay was used as previously described(40-42) to rapidly evaluate the drug efficacy against SARS-CoV-2 infection in Vero E6 and A549 cell cultures.	Vero E6 suggested: None
   The toxicity to the treated cells was assessed by observing floating cells and altered morphology of adhered Vero E6 and A549 cells in wells under the microcopy.	A549 suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)
   Software and Algorithms
   Sentences	Resources
   We used GraphPad 8.0 to analyze the data and used the [Inhibitor] vs. response - Variable slope (four parameters) fitting to determine the values of both IC50 and Hill coefficient.	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. 

   Version published to 10.1101/2020.05.23.112235 on bioRxiv

   May 23, 2020