Recombinant SARS-CoV-2 spike proteins for sero-surveillance and epitope mapping
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Abstract
The newly emergent SARS-CoV-2 coronavirus is closely related to SARS-CoV which emerged in 2002. Studies on coronaviruses in general, and SARS in particular, have identified the virus spike protein (S) as being central to virus tropism, to the generation of a protective antibody response and to the unambiguous detection of past infections. As a result of this centrality SARS-CoV-2 S protein has a role in many aspects of research from vaccines to diagnostic tests. We describe a number of recombinant forms of SARS-CoV-2 S expressed in commonly available expression systems and their preliminary use in diagnostics and epitope mapping. These sources may find use in the current and future analysis of the virus and the Covid-19 disease it causes.
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SciScore for 10.1101/2020.05.21.109298: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature. anti-humansuggested: NoneExperimental Models: Cell Lines Sentences Resources Cell culture: Spodoptera frugiperda (Sf9) and T.niao38 cells were maintained in EX-CELL 420 medium (Sigma) supplemented with 2% fetal bovine serum, 1% penicillin/streptomycin solution, at 27°C with shaking. T.niao38SciScore for 10.1101/2020.05.21.109298: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature. anti-humansuggested: NoneExperimental Models: Cell Lines Sentences Resources Cell culture: Spodoptera frugiperda (Sf9) and T.niao38 cells were maintained in EX-CELL 420 medium (Sigma) supplemented with 2% fetal bovine serum, 1% penicillin/streptomycin solution, at 27°C with shaking. T.niao38suggested: NoneSoftware and Algorithms Sentences Resources Alternate hosts used were SoluBL21 (AMS Biotechnology) and LOBSTR [17]. LOBSTRsuggested: (lobSTR, RRID:SCR_008030)Protein Purification: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading. CytoBuster™suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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