Mechanistic insights into ventricular arrhythmogenesis of hydroxychloroquine and azithromycin for the treatment of COVID-19

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1. 

   ScreenIT

   Jun 9, 2020

   SciScore for 10.1101/2020.05.21.108605: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Study Approval: All procedures were approved by The Institutional Animals Ethics Committees at Henan University, Kaifeng, China under and the national guidelines under which the institution operates, and also conform with the NIH Guide for the Care and Use of Laboratory Animals.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Single-cell electrophysiological recordings: Core human cardiac channels were heterologously expressed in recombinant HEK293 or CHO cell lines and studied using …

   SciScore for 10.1101/2020.05.21.108605: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Study Approval: All procedures were approved by The Institutional Animals Ethics Committees at Henan University, Kaifeng, China under and the national guidelines under which the institution operates, and also conform with the NIH Guide for the Care and Use of Laboratory Animals.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Single-cell electrophysiological recordings: Core human cardiac channels were heterologously expressed in recombinant HEK293 or CHO cell lines and studied using conventional patch clamp methods.	CHO suggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)
   Perforated whole-cell recordings of KCNQ1/E1 and Cav1.2 were obtained following perforation with 200 μg/ml Amphotericin included in the pipette solution.	Cav1.2 suggested: None
   Software and Algorithms
   Sentences	Resources
   All currents were recorded using a MultiClamp 700A amplifier, 1550A digitizer and pCLAMP 10.6 software (Molecular devices, USA).	pCLAMP suggested: (pClamp, RRID:SCR_011323)
   The HCQ structure was obtained from PubChem and prepared for docking using LigPrep.	PubChem suggested: (PubChem, RRID:SCR_004284) LigPrep suggested: (Ligprep, RRID:SCR_016746)
   Poses from Glide, FlexX, and AutoDock Vina were also re-scored using RF-Score-VS.	FlexX suggested: (FlexX, RRID:SCR_000186) AutoDock suggested: (AutoDock, RRID:SCR_012746)
   ECP signals were recorded at a 10 kHz sampling using synchronized 64 channel EMS64-USB-1003 recording systems and recorded using EMapRecord 5.0 software (MapingLab Ltd., UK) and stored in a computer for subsequent off-line analysis.	EMapRecord suggested: None
   The conduction velocity (CV) was calculated from the difference in timing and the known distance between the recording points using EMapScope 5.0 software (MappingLab Ltd.).	EMapScope suggested: None
   The cameras of the optical mapping system, LED lights and stimulator and ECG recording were simultaneously driven by an 8 channel TTL analog-digital converter and OMapRecord 4.0 software (MappingLab Ltd.).	OMapRecord suggested: None
   For the analysis of optical mapping signals and generation of isochronal maps, data were semi-automatically processed using OMapScope 5.0 software (MappingLab Ltd).	OMapScope suggested: None
   Statistical analysis: All patch clamp recorded data were analyzed using Clampfit 10.6 (Molecular Devices, USA), OriginPro 8.0 (Origin Lab, USA) and Adobe Illustrator 10 (Adobe, USA).	OriginPro suggested: None Adobe Illustrator suggested: (Adobe Illustrator, RRID:SCR_010279)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. 

   Version published to 10.1101/2020.05.21.108605 on bioRxiv

   May 21, 2020