Immunization with the receptor–binding domain of SARS-CoV-2 elicits antibodies cross-neutralizing SARS-CoV-2 and SARS-CoV without antibody-dependent enhancement

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   ScreenIT

   Mar 24, 2021

   SciScore for 10.1101/2020.05.21.107565: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Mouse immunization: All the animal experiments in this study were approved by the Institutional Animal Care and Use Committee at the Institut Pasteur of Shanghai.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   For immunofluorescence analysis, cells were fixed in 4% paraformaldehyde, permeabilized by 0.2% Triton X-100 (Thermo Fisher Scientific, USA), and stained overnight at 4°C with an anti-N mouse polyclonal antibody generated in house.	anti-N suggested: None
   The samples were finally incubated with Alexa Fluor …

   SciScore for 10.1101/2020.05.21.107565: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Mouse immunization: All the animal experiments in this study were approved by the Institutional Animal Care and Use Committee at the Institut Pasteur of Shanghai.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   For immunofluorescence analysis, cells were fixed in 4% paraformaldehyde, permeabilized by 0.2% Triton X-100 (Thermo Fisher Scientific, USA), and stained overnight at 4°C with an anti-N mouse polyclonal antibody generated in house.	anti-N suggested: None
   The samples were finally incubated with Alexa Fluor 488-labeled donkey anti-mouse IgG secondary antibody (1:1000, Thermo Fisher Scientific) at 37°C for 1 hour.	anti-mouse IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   HEK 293T, A20, THP-1, and K562 cells were purchased from the Cell Bank of Chinese Academy of Sciences (www.cellbank.org.cn).	THP-1 suggested: None K562 suggested: NCI-DTP Cat# K-562, RRID:CVCL_0004)
   Cell-cell fusion inhibition assay: HEK 293T cells were separately transfected with a plasmid encoding the SARS-CoV-2 S:EGFP fusion protein (pcDNA-S:EGFP) or with a plasmid encoding the hACE2:mCherry fusion protein (pcDNA-hACE2:mCherry).	HEK 293T suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
   The mixture was then onto VeroE6 cells overexpressing hACE2 (denoted as VeroE6-hACE2) preseeded in 48-well plates.	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Two groups of six BALB/c mice were injected intraperitoneally (i.p.) with the RBD vaccine and the control antigen, respectively, at days 0, 10, and 25.	BALB/c suggested: None
   Software and Algorithms
   Sentences	Resources
   Statistics analysis: All statistical analyses were performed using GraphPad Prism software v5.0. Kaplan–Meier survival curves were compared using log-rank test.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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2. 

   Version published to 10.1101/2020.05.21.107565 on bioRxiv

   May 21, 2020