SARS-CoV-2 RNA concentrations in primary municipal sewage sludge as a leading indicator of COVID-19 outbreak dynamics
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Abstract
We report a time course of SARS-CoV-2 RNA concentrations in primary sewage sludge during the Spring COVID-19 outbreak in a northeastern U.S. metropolitan area. SARS-CoV-2 RNA was detected in all environmental samples, and when adjusted for the time lag, the virus RNA concentrations tracked the COVID-19 epidemiological curve. SARS-CoV-2 RNA concentrations were a leading indicator of community infection ahead of compiled COVID-19 testing data and local hospital admissions. Decisions to implement or relax public health measures and restrictions require timely information on outbreak dynamics in a community.
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SciScore for 10.1101/2020.05.19.20105999: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Samples were analyzed using the Bio-Rad iTaq Universal Probes One-Step kit in 20 μL reactions run at 50°C for 10 min, 95°C for 1 min followed by 40 cycles of 95°C for 10 s and 60°C for 30 s per the manufacturer’s recommendations. Bio-Rad iTaq Universal Probessuggested: NoneiTaqsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecog…SciScore for 10.1101/2020.05.19.20105999: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Samples were analyzed using the Bio-Rad iTaq Universal Probes One-Step kit in 20 μL reactions run at 50°C for 10 min, 95°C for 1 min followed by 40 cycles of 95°C for 10 s and 60°C for 30 s per the manufacturer’s recommendations. Bio-Rad iTaq Universal Probessuggested: NoneiTaqsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Jurisdictions can use primary sludge SARS-CoV-2 concentrations to preempt community outbreak dynamics or provide an additional basis for easing restrictions, especially when there are limitations in clinical testing. Raw wastewater and sludge-based surveillance is particularly useful for low and middle-income countries where clinical testing capacity is limited.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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