Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in Saccharomyces cerevisiae as a vaccine candidate

  1. Kajal Arora
  2. Ruchir Rastogi
  3. Nupur Mehrotra Arora
  4. Deepak Parashar
  5. Jeny Paliwal
  6. Aelia Naqvi
  7. Apoorva Srivastava
  8. Sudhir Kumar Singh
  9. Sriganesh Kalyanaraman
  10. Swaroop Potdar
  11. Devanand Kumar
  12. Vidya Bhushan Arya
  13. Sarthi Bansal
  14. Satabdi Rautray
  15. Indrajeet Singh
  16. Pankaj Surendra Fengade
  17. Bibekanand Kumar
  18. Prabuddha Kumar Kundu

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Abstract

Spike, Envelope and Membrane proteins from the SARS CoV-2 virus surface coat are important vaccine targets. We hereby report recombinant co-expression of the three proteins (Spike, Envelope and Membrane) in a engineered Saccharomyces cerevisiae platform (D-Crypt™) and their self-assembly as Virus-like particle (VLP). This design as a multi-antigenic VLP for SARS CoV-2 has the potential to be a scalable vaccine candidate. The VLP is confirmed by transmission electron microscopy (TEM) images of the SARS CoV-2, along with supportive HPLC, Dynamic Light Scattering (DLS) and allied analytical data. The images clearly outline the presence of a “Corona” like morphology, and uniform size distribution.

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  1. ScreenIT

    SciScore for 10.1101/2020.05.18.099234: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Samples corresponding to 1.4 OD600 of cells were resolved over SDS-Western blot was developed using anti-His specific antibody (Cat no. Sigma:H1029) at a dilution of 1:3000 and HRP conjugated anti-mouse secondary antibody (Anti mouse IgG-A4416) at dilution of 1:4000.
    anti-His
    suggested: (SignalChem Cat# H99-63R-100, RRID:AB_1537505)
    anti-mouse
    suggested: (Sigma-Aldrich Cat# A4416, RRID:AB_258167)
    Anti mouse IgG-A4416
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1101/2020.05.18.099234v1 on bioRxiv