Identification of five antiviral compounds from the Pandemic Response Box targeting SARS-CoV-2
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Abstract
With currently over 4 million confirmed cases worldwide, including more than 300’000 deaths, the current Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has a major impact on the economy and health care system. Currently, a limited amount of prophylactic or therapeutic intervention options are available against SARS-CoV-2. In this study, we screened 400 compounds from the antimicrobial ‘Pandemic Response Box’ library for inhibiting properties against SARS-CoV-2. We identified sixteen compounds that potently inhibited SARS-CoV-2 replication, of which five compounds displayed equal or even higher antiviral activity compared to Remdesivir. These results show that five compounds should be further investigated for their mode of action, safety and efficacy against SARS-CoV-2.
Highlights
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400 compounds from the pandemic response box were tested for antiviral activity against SARS-CoV-2.
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5 compounds had an equal or higher antiviral efficacy towards SARS-CoV-2, compared to the nucleoside analogue Remdesivir.
Article activity feed
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SciScore for 10.1101/2020.05.17.100404: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources IC50 determination of selected compounds: Vero-E6 cells were seeded in 96-well clear bottom, black plates (Costar), 20’000 cells per well one day prior to the experiment. Vero-E6suggested: NoneExperimental Models: Organisms/Strains Sentences Resources SARS-CoV-2 (SARS-CoV-2/München-1.1/2020/929 [15], stocks were produced on Vero-E6 cells, aliquoted and stored at - 80°C. SARS-CoV-2 (SARS-CoV-2/München-1.1/2020/929suggested: None… SciScore for 10.1101/2020.05.17.100404: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources IC50 determination of selected compounds: Vero-E6 cells were seeded in 96-well clear bottom, black plates (Costar), 20’000 cells per well one day prior to the experiment. Vero-E6suggested: NoneExperimental Models: Organisms/Strains Sentences Resources SARS-CoV-2 (SARS-CoV-2/München-1.1/2020/929 [15], stocks were produced on Vero-E6 cells, aliquoted and stored at - 80°C. SARS-CoV-2 (SARS-CoV-2/München-1.1/2020/929suggested: NoneSoftware and Algorithms Sentences Resources Four images per well were acquired to cover the entire surface of the well and processed and stitched using the Gen5 Image prime software package (v3.08.01). Gen5suggested: (Gen5, RRID:SCR_017317)Data representation: Graphs were generated using GraphPad Prism software version 8.4.2 and the final figures were assembled in Adobe Illustrator CS6. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Adobe Illustratorsuggested: (Adobe Illustrator, RRID:SCR_010279)Brightness and contrast of microscopy picture were minimally adjusted and processed identically to their corresponding control using FIJI. FIJIsuggested: (Fiji, RRID:SCR_002285)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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