Identification of potential treatments for COVID-19 through artificial intelligence-enabled phenomic analysis of human cells infected with SARS-CoV-2
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Abstract
To identify potential therapeutic stop-gaps for SARS-CoV-2, we evaluated a library of 1,670 approved and reference compounds in an unbiased, cellular image-based screen for their ability to suppress the broad impacts of the SARS-CoV-2 virus on phenomic profiles of human renal cortical epithelial cells using deep learning. In our assay, remdesivir is the only antiviral tested with strong efficacy, neither chloroquine nor hydroxychloroquine have any beneficial effect in this human cell model, and a small number of compounds not currently being pursued clinically for SARS-CoV-2 have efficacy. We observed weak but beneficial class effects of β-blockers, mTOR/PI3K inhibitors and Vitamin D analogues and a mild amplification of the viral phenotype with β-agonists.
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SciScore for 10.1101/2020.04.21.054387: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources ” SARS-CoV-2 propagation and controls: The USA-WA1/2020 strain of SARS-CoV-2 was propagated in Vero 76 cells (ATCC CRL-1587). Vero 76suggested: NoneViral inactivation in this sample was verified using visual CPE on Vero cells, where undetectable level of active virus was observed. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)Caco-2 cells (HTB-37) were acquired from ATCC and propagated at 37°C with 5% CO2 in EMEM + 10% FBS. Cac…SciScore for 10.1101/2020.04.21.054387: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources ” SARS-CoV-2 propagation and controls: The USA-WA1/2020 strain of SARS-CoV-2 was propagated in Vero 76 cells (ATCC CRL-1587). Vero 76suggested: NoneViral inactivation in this sample was verified using visual CPE on Vero cells, where undetectable level of active virus was observed. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)Caco-2 cells (HTB-37) were acquired from ATCC and propagated at 37°C with 5% CO2 in EMEM + 10% FBS. Caco-2suggested: NoneSoftware and Algorithms Sentences Resources Plates were imaged using Image Express Micro Confocal High-Content Imaging System ( Image Expresssuggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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