Multiscale 3-dimensional pathology findings of COVID-19 diseased lung using high-resolution cleared tissue microscopy
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
The study of pulmonary samples from individuals who have died as a direct result of COVID-19 infection is vital to our understanding of the pathogenesis of this disease. Histopathologic studies of lung tissue from autopsy of patients with COVID-19 specific mortality are only just emerging. All existing reports have relied on traditional 2-dimensional slide-based histological methods for specimen preparation. However, emerging methods for high-resolution, massively multiscale imaging of tissue microstructure using fluorescence labeling and tissue clearing methods enable the acquisition of tissue histology in 3-dimensions, that could open new insights into the nature of SARS-Cov-2 infection and COVID-19 disease processes. In this article, we present the first 3-dimensional images of lung autopsy tissues taken from a COVID-19 patient, including 3D “virtual histology” of cubic-millimeter volumes of the diseased lung, providing unique insights into disease processes contributing to mortality that could inform frontline treatment decisions.
Article activity feed
-
-
SciScore for 10.1101/2020.04.11.037473: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All experiments were conducted in accordance with SARS-CoV-2 (Risk Factor 3)-specific protocols approved by the Tulane University Institutional Biosafety Committee. Randomization not detected. Blinding not detected. Power Analysis 8 The system is fitted with a 488-nm laser source (Omicron, PhoxX; maximum power = 200 mW) for fluorescence excitation of Eosin and a 647-nm laser source (Omicron, PhoxX; maximum power = 140 mW) for fluorescence excitation of TO-PRO-3 iodide. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources After regions of interest were identified, sub-volumes were loaded at full resolution into Amira … SciScore for 10.1101/2020.04.11.037473: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All experiments were conducted in accordance with SARS-CoV-2 (Risk Factor 3)-specific protocols approved by the Tulane University Institutional Biosafety Committee. Randomization not detected. Blinding not detected. Power Analysis 8 The system is fitted with a 488-nm laser source (Omicron, PhoxX; maximum power = 200 mW) for fluorescence excitation of Eosin and a 647-nm laser source (Omicron, PhoxX; maximum power = 140 mW) for fluorescence excitation of TO-PRO-3 iodide. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources After regions of interest were identified, sub-volumes were loaded at full resolution into Amira and the OpenViSUS17 interactive framework for further visualization. Amirasuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
-