Molecular Basis for ADP-ribose Binding to the Macro-X Domain of SARS-CoV-2 Nsp3
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Abstract
The virus that causes COVID-19, SARS-CoV-2, has a large RNA genome that encodes numerous proteins that might be targets for antiviral drugs. Some of these proteins, such as the RNA-dependent RNA polymers, helicase and main protease, are well conserved between SARS-CoV-2 and the original SARS virus, but several others are not. This study examines one of the proteins encoded by SARS-CoV-2 that is most different, a macrodomain of nonstructural protein 3 (nsp3). Although 26% of the amino acids in this SARS-CoV-2 macrodomain differ from those seen in other coronaviruses, biochemical and structural data reveal that the protein retains the ability to bind ADP-ribose, which is an important characteristic of beta coronaviruses, and potential therapeutic target.
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SciScore for 10.1101/2020.03.31.014639: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources Concentration of the purified protein was determined by measuring absorbance at 260 nm using a molar extinction coefficient of 10,555 M-1 cm-1, which was calculated with the ProtParam tool (https://web.expasy.org/protparam/). ProtParamsuggested: (ProtParam Tool, RRID:SCR_018087)The data were indexed and integrated with DIALS 7, 8 as implemented in version 7.2 of the CCP4 software suite.9, 10 Data scaling and reduction was performed using AIMLESS.11–13 Data collection statistics are provided in Table 1. CCP4suggested: (CCP4, RRID:SCR_007255)Results from OddPub: Thank you for sharing your data.
SciScore for 10.1101/2020.03.31.014639: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources Concentration of the purified protein was determined by measuring absorbance at 260 nm using a molar extinction coefficient of 10,555 M-1 cm-1, which was calculated with the ProtParam tool (https://web.expasy.org/protparam/). ProtParamsuggested: (ProtParam Tool, RRID:SCR_018087)The data were indexed and integrated with DIALS 7, 8 as implemented in version 7.2 of the CCP4 software suite.9, 10 Data scaling and reduction was performed using AIMLESS.11–13 Data collection statistics are provided in Table 1. CCP4suggested: (CCP4, RRID:SCR_007255)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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