Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2

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1. 

   ScreenIT

   Jun 5, 2020

   SciScore for 10.1101/2020.03.16.20035014: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   This study was approved by the Hospital Ethics Committee of the General Hospital of the Central Theater Command of the PLA ([2020]003-1) and the written informed consent was waived for emerging infectious diseases. rN-based ELISA: The recombinant nucleocapsid (rN) protein-based ELISA kit (Lizhu, Zhuhai, China) was used for the detection of IgM or IgG antibody against SARS-CoV-2.	SARS-CoV-2 suggested: None
   For IgM detection, ELISA plates were coated with monoclonal mouse anti-human IgM (μ …

   SciScore for 10.1101/2020.03.16.20035014: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   This study was approved by the Hospital Ethics Committee of the General Hospital of the Central Theater Command of the PLA ([2020]003-1) and the written informed consent was waived for emerging infectious diseases. rN-based ELISA: The recombinant nucleocapsid (rN) protein-based ELISA kit (Lizhu, Zhuhai, China) was used for the detection of IgM or IgG antibody against SARS-CoV-2.	SARS-CoV-2 suggested: None
   For IgM detection, ELISA plates were coated with monoclonal mouse anti-human IgM (μ chain) antibody.	anti-human IgM (μ chain) suggested: None
   After incubation and washing, HRP-conjugated monoclonal mouse anti-human IgG antibody was added to the plates for detection.	anti-human IgG suggested: None
   After five wash steps with washing buffer, 100 μL of diluted HRP-conjugated anti-human IgM antibodies was added to the wells, and samples were incubated at 37 °C for 30 min.	anti-human IgM suggested: None
   Software and Algorithms
   Sentences	Resources
   Statistical analyses were performed using SPSS version 22.0.	SPSS suggested: (SPSS, RRID:SCR_002865)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. Version published to 10.1128/jcm.00461-20

   May 26, 2020
3. 

   Version published to 10.1101/2020.03.16.20035014v1 on medRxiv

   Mar 20, 2020