Viral Architecture of SARS-CoV-2 with Post-Fusion Spike Revealed by Cryo-EM
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Abstract
Since December 2019, the outbreak of Coronavirus Disease 2019 (COVID-19) spread from Wuhan, China to the world, it has caused more than 87,000 diagnosed cases and more than 3,000 deaths globally. To fight against COVID-19, we carried out research for the near native SARS-CoV-2 and report here our preliminary results obtained. The pathogen of the COVID-19, the native SARS-CoV-2, was isolated, amplified and purified in a BSL-3 laboratory. The whole viral architecture of SARS-CoV-2 was examined by transmission electron microscopy (both negative staining and cryo-EM). We observed that the virion particles are roughly spherical or moderately pleiomorphic. Spikes have nail-like shape towards outside with a long body embedded in the envelope. The morphology of virion observed in our result indicates that the S protein of SARS-CoV-2 is in post-fusion state, with S1 disassociated. This state revealed by cryo-EM first time could provide an important information for the identification and relevant clinical research of this new coronavirus.
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SciScore for 10.1101/2020.03.02.972927: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After three washes, 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody solution (Sangon Biotech) was added to each well and incubated at 37°C for 60 minutes. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Vero, LLC-MK2 and Huh-7 cells were used for the virus isolation in the biosafety level 3 (BSL-3) laboratory. Huh-7suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)Virus Preparation: Virus was … SciScore for 10.1101/2020.03.02.972927: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After three washes, 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody solution (Sangon Biotech) was added to each well and incubated at 37°C for 60 minutes. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Vero, LLC-MK2 and Huh-7 cells were used for the virus isolation in the biosafety level 3 (BSL-3) laboratory. Huh-7suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)Virus Preparation: Virus was amplified in Vero cell in an incubator at 37°C and 5% CO2 for 96 hours and the viral load was determined by qPCR assay. Verosuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
- No funding statement was detected.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.03.02.972927: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement This study received approval from the Research Ethics Committee of Shenzhen Third People's Hospital, China (approval number: 2020-038). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Results and Discussion A 62-year-old male was admitted to our hospital on 15 January, 2020 with pneumonia, and was further diagnosed as COVID-19. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After three washes , 100 µl of horseradish peroxidase ( HRP)-conjugated goat anti-human IgG antibody solution ( Sangon Biotech ) was added to each well and incubated at 37°C for 60 minutes . …SciScore for 10.1101/2020.03.02.972927: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement This study received approval from the Research Ethics Committee of Shenzhen Third People's Hospital, China (approval number: 2020-038). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Results and Discussion A 62-year-old male was admitted to our hospital on 15 January, 2020 with pneumonia, and was further diagnosed as COVID-19. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After three washes , 100 µl of horseradish peroxidase ( HRP)-conjugated goat anti-human IgG antibody solution ( Sangon Biotech ) was added to each well and incubated at 37°C for 60 minutes . anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Typical cytopathic effects ( CPE ) were observed at 4 days post inoculation ( dpi ) in Vero cell , including cell rounding , shrinkage , lysis , and detachment throughout the cell monolayers ( Figure 1A) . Verosuggested: NoneVero , LLC-MK2 and Huh-7 cells were used for the virus isolation in the biosafety level 3 ( BSL-3 ) laboratory . Huh-7suggested: CLS Cat# 300156/p7178_HuH7, CVCL_0336Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
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SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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