Predictions for the binding domain and potential new drug targets of 2019-nCoV
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Abstract
An outbreak of new SARS-like viral in Wuhan, China has been named 2019-nCoV. The current state of the epidemic is increasingly serious, and there has been the urgent necessity to develop an effective new drug. In previous studies, it was found that the conformation change in CTD1 was the region where SARS-CoV bound to human ACE2. Although there are mutations of the 2019-nCoV, the binding energy of ACE2 remains high. The surface glycoprotein of 2019-nCoV was coincident with the CTD1 region of the S-protein by comparing the I-TASSER prediction model with the actual SARS model, which suggests that 2019-nCoV may bind to the ACE2 receptor through conformational changes. Furthermore, site prediction on the surface glycoprotein of 2019-nCoV suggests some core amino acid area may be a novel drug target against 2019-nCoV.
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SciScore for 10.1101/2020.02.26.961938: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources Analysis of Virus Variability: Using online tools blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi) to analyse MN908947.3 amino acid sequence and the 33 2019-nCoV sequences downloaded from GISAID. https://blast.ncbi.nlm.nih.gov/Blast.cgisuggested: (TBLASTX, RRID:SCR_011823)Prediction by I-TASSER Protein Model: The MN908947.3 spike protein sequence was first extracted from GenBank file, the sequence was then submitted to the online tools (https://zhanglab.ccmb.med.umich.edu/I-TASSER/) which uses existing model to predict protein structure. https://zhanglab.ccmb.med.umich.edu/I-TASSER/suggested…SciScore for 10.1101/2020.02.26.961938: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources Analysis of Virus Variability: Using online tools blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi) to analyse MN908947.3 amino acid sequence and the 33 2019-nCoV sequences downloaded from GISAID. https://blast.ncbi.nlm.nih.gov/Blast.cgisuggested: (TBLASTX, RRID:SCR_011823)Prediction by I-TASSER Protein Model: The MN908947.3 spike protein sequence was first extracted from GenBank file, the sequence was then submitted to the online tools (https://zhanglab.ccmb.med.umich.edu/I-TASSER/) which uses existing model to predict protein structure. https://zhanglab.ccmb.med.umich.edu/I-TASSER/suggested: (I-TASSER, RRID:SCR_014627)Results from OddPub: Thank you for sharing your code and data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 7. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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